Literature DB >> 4073506

A convenient S-2-aminoethylation of cysteinyl residues in reduced proteins.

K Okazaki, H Yamada, T Imoto.   

Abstract

The cysteinyl residues in proteins had been S-2-aminoethylated with ethylenimine quantitatively. However, ethylenimine is now listed as a carcinogen and is no longer commercially available. A method of converting cysteinyl residues to S-2-aminoethyl derivatives quantitatively using 2-bromoethylamine under mild conditions was developed here.

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Year:  1985        PMID: 4073506     DOI: 10.1016/0003-2697(85)90607-4

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  9 in total

1.  Expressed protein ligation at methionine: N-terminal attachment of homocysteine, ligation, and masking.

Authors:  Tomohiro Tanaka; Anne M Wagner; John B Warner; Yanxin J Wang; E James Petersson
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2.  Unusual fragmentation of derivatized cysteine-containing peptides.

Authors:  Nick Degraan-Weber; Bingqing Zhao; James P Reilly
Journal:  Rapid Commun Mass Spectrom       Date:  2018-09-15       Impact factor: 2.419

3.  Purification and biochemical characterization of an organic-solvent-tolerant thioredoxin from dromedary pancreas.

Authors:  Abir G Ben Bacha; Hafedh Mejdoub
Journal:  Protein J       Date:  2012-01       Impact factor: 2.371

4.  Novel organic hydroperoxide-sensing and responding mechanisms for OhrR, a major bacterial sensor and regulator of organic hydroperoxide stress.

Authors:  Warunya Panmanee; Paiboon Vattanaviboon; Leslie B Poole; Skorn Mongkolsuk
Journal:  J Bacteriol       Date:  2006-02       Impact factor: 3.490

5.  The complete amino acid sequence of bovine antithrombin (ATIII).

Authors:  H Mejdoub; M Le Ret; Y Boulanger; M Maman; J Choay; J Reinbolt
Journal:  J Protein Chem       Date:  1991-04

6.  The complete amino acid sequence of ribosomal protein S8 from Thermus thermophilus.

Authors:  J Reinbolt; I Eliseikina; S Sedelnilkova; M Garber; C Ehresmann; B Ehresmann
Journal:  J Protein Chem       Date:  1993-02

7.  Biochemical properties of pancreatic colipase from the common stingray Dasyatis pastinaca.

Authors:  Abir Ben Bacha; Aida Karray; Lobna Daoud; Emna Bouchaala; Madiha Bou Ali; Youssef Gargouri; Yassine Ben Ali
Journal:  Lipids Health Dis       Date:  2011-05-08       Impact factor: 3.876

8.  Purification and biochemical characterization of pancreatic phospholipase A2 from the common stingray Dasyatis pastinaca.

Authors:  Abir Ben Bacha; Aida Karray; Emna Bouchaala; Youssef Gargouri; Yassine Ben Ali
Journal:  Lipids Health Dis       Date:  2011-02-17       Impact factor: 3.876

9.  A high-throughput peptidomic strategy to decipher the molecular diversity of cyclic cysteine-rich peptides.

Authors:  Aida Serra; Xinya Hemu; Giang K T Nguyen; Ngan T K Nguyen; Siu Kwan Sze; James P Tam
Journal:  Sci Rep       Date:  2016-03-11       Impact factor: 4.379

  9 in total

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