Literature DB >> 40720

A fluorimetric assay of the phosphoribosylation of 6-mercaptopurine in human blood cells.

P C Wong, Y Hey, J S Fowler.   

Abstract

A new fluorimetric method to assay HGPRT (hypoxanthine-guanine phosphoribosyl transferase, EC 2.4.2.8) from human red cell lysates using 6MP (6-mercaptopurine) as substrate is described. This assay is compared with an existing spectrophotometric method using hypoxanthine as substrate. Precision, sensitivity limits and kinetic differences of these assays are reported.

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Year:  1979        PMID： 40720     DOI： 10.1016/0009-8981(79)90138-4

Source DB:  PubMed          Journal:  Clin Chim Acta        ISSN： 0009-8981            Impact factor:   3.786


  1 in total

1.  Red blood cell hypoxanthine phosphoribosyltransferase activity measured using 6-mercaptopurine as a substrate: a population study in children with acute lymphoblastic leukaemia.

Authors:  L Lennard; J P Hale; J S Lilleyman
Journal:  Br J Clin Pharmacol       Date:  1993-10       Impact factor: 4.335
  1 in total

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