Literature DB >> 4070962

Purification of the pepsinogen A isozymogens by means of high resolution ion-exchange chromatography. Evidence for post-translational modifications.

J Défize, G Pals, J C Pronk, R R Frants, G Rimmelzwaan, B D Westerveld, A W Eriksson.   

Abstract

Total human pepsinogen (PG) was isolated from gastric fundic mucosa and PGA (formerly called PGI) from urine, using standard ion-exchange and gel filtration techniques. Gastric PGA was separated from PGC (formerly called PGII) either by immunoaffinity or high resolution ion-exchange chromatography (fast protein liquid chromatography, Pharmacia, Uppsala, Sweden). The individual PGA isozymogens 2, 3, 4 and 5 could be isolated to homogeneity with the aid of the same ion-exchanger. Evidence was obtained for the existence of secondary modifications of the PGA fractions 3, 4 and 5, electrophoretically overlapping the primary (genetic) isozymogens.

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Year:  1985        PMID： 4070962     DOI： 10.3109/00365518509155273

Source DB:  PubMed          Journal:  Scand J Clin Lab Invest        ISSN： 0036-5513            Impact factor:   1.713


  3 in total

1.  Immunoblot technique to visualise serum pepsinogen A isozymogen patterns.

Authors:  A Zwiers; C Toonstra; G Pals; A J Donker; S G Meuwissen; R W ten Kate
Journal:  J Clin Pathol       Date:  1990-10       Impact factor: 3.411

2.  Molecular cloning of a pair of human pepsinogen A genes which differ by a Glu----Lys mutation in the activation peptide.

Authors:  M P Evers; B Zelle; D S Peeper; W H Mager; R J Planta; A W Eriksson; R R Frants
Journal:  Hum Genet       Date:  1987-10       Impact factor: 4.132

3.  Discrepancies between gastric mucosal and urinary pepsinogen A patterns and in vitro synthesis and secretion of human pepsinogen.

Authors:  G Pals; B D Westerveld; J Defize; J C Pronk; H Brand; M Flipse; C Verwey; S G Meuwissen; A W Eriksson
Journal:  Dig Dis Sci       Date:  1988-02       Impact factor: 3.199
  3 in total

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