Literature DB >> 4066920

Comparison of ortho respiratory syncytial virus enzyme-linked immunosorbent assay and HEp-2 cell culture.

K Bromberg, G Tannis, B Daidone, L Clarke, M F Sierra.   

Abstract

Two hundred seventy nasopharyngeal aspirates were tested in duplicate with the Ortho Diagnostics, Inc. (Raritan, N.J.), respiratory syncytial virus antigen enzyme-linked immunosorbent assay. The test was sensitive (80 to 82%) and specific (96%) when compared with cell culture. The enzyme-linked immunosorbent assay detected seven antigen-positive specimens not among the 71 specimens that were positive for respiratory syncytial virus in cell culture. A blocking test confirmed those specimens as true positives (specificity, 100%).

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Year:  1985        PMID： 4066920      PMCID： PMC271886          DOI： 10.1128/jcm.22.6.1071-1072.1985

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  7 in total

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Authors:  C B Hall; R G Douglas
Journal:  J Infect Dis       Date:  1975-01       Impact factor: 5.226

2.  Epidemiology of acute lower respiratory disease in children.

Authors:  P Glezen; F W Denny
Journal:  N Engl J Med       Date:  1973-03-08       Impact factor: 91.245

3.  Rapid diagnosis of respiratory syncytial virus infection in children by the immunofluorescent technique.

Authors:  J E Cradock-Watson; J McQuillin; P S Gardner
Journal:  J Clin Pathol       Date:  1971-05       Impact factor: 3.411

4.  Monoclonal antibodies to respiratory syncytial virus: detection of virus neutralization and other antigen-antibody systems using infected human and murine cells.

Authors:  P J Cote; B F Fernie; E C Ford; J W Shih; J L Gerin
Journal:  J Virol Methods       Date:  1981-10       Impact factor: 2.014

5.  Enzyme-linked immunosorbent assay for detection of respiratory syncytial virus infection: application to clinical samples.

Authors:  K McIntosh; R M Hendry; M L Fahnestock; L T Pierik
Journal:  J Clin Microbiol       Date:  1982-08       Impact factor: 5.948

6.  Nosocomial viral infections: I. Epidemiology and significance.

Authors:  W M Valenti; M A Menegus; C B Hall; P H Pincus; R G Douglas
Journal:  Infect Control       Date:  1980 Jan-Feb

7.  Aerosolized ribavirin treatment of infants with respiratory syncytial viral infection. A randomized double-blind study.

Authors:  C B Hall; J T McBride; E E Walsh; D M Bell; C L Gala; S Hildreth; L G Ten Eyck; W J Hall
Journal:  N Engl J Med       Date:  1983-06-16       Impact factor: 91.245
  7 in total
  16 in total

1.  Comparison of two rapid methods for detection of respiratory syncytial virus (RSV) (Testpack RSV and ortho RSV ELISA) with direct immunofluorescence and virus isolation for the diagnosis of pediatric RSV infection.

Authors:  E E Thomas; L E Book
Journal:  J Clin Microbiol       Date:  1991-03       Impact factor: 5.948

Review 2.  Pertussis: the disease and new diagnostic methods.

Authors:  R L Friedman
Journal:  Clin Microbiol Rev       Date:  1988-10       Impact factor: 26.132

3.  An enzyme-linked immunosorbent assay using monoclonal antibodies for the detection of respiratory syncytial virus in clinical specimens.

Authors:  G Obert; C Beyer
Journal:  Arch Virol       Date:  1988       Impact factor: 2.574

4.  Comparison of three enzyme-linked immunosorbent assays and a direct fluorescent-antibody test for detection of respiratory syncytial virus antigen.

Authors:  L R Krilov; L Marcoux; H D Isenberg
Journal:  J Clin Microbiol       Date:  1988-02       Impact factor: 5.948

5.  Comparison of three rapid diagnostic techniques for detection of respiratory syncytial virus from nasal wash specimens.

Authors:  T Chonmaitree; B J Bessette-Henderson; R E Hepler; H L Lucia
Journal:  J Clin Microbiol       Date:  1987-04       Impact factor: 5.948

Review 6.  Detection, pathogenesis, and therapy of respiratory syncytial virus infections.

Authors:  R C Welliver
Journal:  Clin Microbiol Rev       Date:  1988-01       Impact factor: 26.132

7.  Comparison of two new tests for rapid diagnosis of respiratory syncytial virus infections by enzyme-linked immunosorbent assay and immunofluorescence techniques.

Authors:  F Freymuth; M Quibriac; J Petitjean; M L Amiel; P Pothier; A Denis; J F Duhamel
Journal:  J Clin Microbiol       Date:  1986-12       Impact factor: 5.948

8.  Evaluation of the Abbott TESTPACK RSV enzyme immunoassay for detection of respiratory syncytial virus in nasopharyngeal swab specimens.

Authors:  E M Swierkosz; R Flanders; L Melvin; J D Miller; M W Kline
Journal:  J Clin Microbiol       Date:  1989-06       Impact factor: 5.948

9.  Nasopharyngeal swabs and nasopharyngeal aspirates equally effective for the diagnosis of viral respiratory disease in hospitalized children.

Authors:  H Frayha; S Castriciano; J Mahony; M Chernesky
Journal:  J Clin Microbiol       Date:  1989-06       Impact factor: 5.948

10.  Comparison of HEp-2 cell culture and Abbott respiratory syncytial virus enzyme immunoassay.

Authors:  K Bromberg; G Tannis; B Daidone; L Clarke; M Sierra
Journal:  J Clin Microbiol       Date:  1987-02       Impact factor: 5.948
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