| Literature DB >> 4066644 |
M Kito, H Takamura, H Narita, R Urade.
Abstract
A simple and sensitive method was developed for quantitative analysis of phospholipid molecular species. Diacylglycerols were prepared from phospholipids by phospholipase C treatment and converted to the corresponding dinitrobenzoyl derivatives, which could be sensitively detected at 254 nm. The derivatives of 21 molecular species were resolved by high-performance liquid chromatography with an octadecylsilyl reversed-phase column. All the derivatives had the same peak area per mol, and peak areas were proportional to the amounts of the derivatives. Quantification was carried out at the picomole level.Entities:
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Year: 1985 PMID： 4066644 DOI： 10.1093/oxfordjournals.jbchem.a135285
Source DB: PubMed Journal: J Biochem ISSN： 0021-924X Impact factor: 3.387