Literature DB >> 4065095

The phalloidin binding site of F-actin.

J Vandekerckhove, A Deboben, M Nassal, T Wieland.   

Abstract

Tritium-containing affinity-labelling derivatives of phalloidin, an alkylating iodoacetyl compound (EAL) and a photolabile, carbene generating diazirine (PAL), have been reacted with rabbit muscle actin, the former after protection of thiol groups with N-ethylmaleimide. Labelled peptides generated by tryptic and/or thermolysin digestion were isolated by paper peptide mapping and characterized by determination of their amino acid sequences. EAL binds to methionine-119 and methionine-355; PAL binds to glutamic acid-117. These residues are located in regions with extremely conserved amino acid sequences. The cleft between the two domains of the actin monomer is suggested as the possible binding site for phalloidin.

Entities:  

Mesh：

Substances：

Year:  1985        PMID： 4065095      PMCID： PMC554583          DOI： 10.1002/j.1460-2075.1985.tb04008.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  41 in total

1.  Actin amino-acid sequences. Comparison of actins from calf thymus, bovine brain, and SV40-transformed mouse 3T3 cells with rabbit skeletal muscle actin.

Authors:  J Vandekerckhove; K Weber
Journal:  Eur J Biochem       Date:  1978-10-16

2.  The amino acid sequence of actin from chicken skeletal muscle actin and chicken gizzard smooth muscle actin.

Authors:  J Vandekerckhove; K Weber
Journal:  FEBS Lett       Date:  1979-06-15       Impact factor: 4.124

3.  Cytoplasmic streaming in Amoeba proteus is inhibited by the actin-specific drug phalloidin.

Authors:  J Wehland; W Stockem; K Weber
Journal:  Exp Cell Res       Date:  1978-09       Impact factor: 3.905

4.  The amino acid sequence of Physarum actin.

Authors:  J Vandekerckhove; K Weber
Journal:  Nature       Date:  1978-12-14       Impact factor: 49.962

5.  A gas-liquid solid phase peptide and protein sequenator.

Authors:  R M Hewick; M W Hunkapiller; L E Hood; W J Dreyer
Journal:  J Biol Chem       Date:  1981-08-10       Impact factor: 5.157

6.  Complete nucleotide sequence of a soybean actin gene.

Authors:  D M Shah; R C Hightower; R B Meagher
Journal:  Proc Natl Acad Sci U S A       Date:  1982-02       Impact factor: 11.205

7.  The primary structure of actin from rabbit skeletal muscle. Completion and analysis of the amino acid sequence.

Authors:  J H Collins; M Elzinga
Journal:  J Biol Chem       Date:  1975-08-10       Impact factor: 5.157

8.  Phalloidin-induced actin polymerization in the cytoplasm of cultured cells interferes with cell locomotion and growth.

Authors:  J Wehland; M Osborn; K Weber
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

9.  Nucleotide sequence of the rat skeletal muscle actin gene.

Authors:  R Zakut; M Shani; D Givol; S Neuman; D Yaffe; U Nudel
Journal:  Nature       Date:  1982-08-26       Impact factor: 49.962

10.  Three-dimensional structure of the complex of skeletal muscle actin and bovine pancreatic DNAse I at 6-A resolution.

Authors:  D Suck; W Kabsch; H G Mannherz
Journal:  Proc Natl Acad Sci U S A       Date:  1981-07       Impact factor: 11.205
View more
  34 in total

1.  Role of actin cortex in the subplasmalemmal transport of secretory granules in PC-12 cells.

Authors:  T Lang; I Wacker; I Wunderlich; A Rohrbach; G Giese; T Soldati; W Almers
Journal:  Biophys J       Date:  2000-06       Impact factor: 4.033

2.  Modulation of actin filament behavior by GAP-43 (neuromodulin) is dependent on the phosphorylation status of serine 41, the protein kinase C site.

Authors:  Q He; E W Dent; K F Meiri
Journal:  J Neurosci       Date:  1997-05-15       Impact factor: 6.167

3.  Dual pools of actin at presynaptic terminals.

Authors:  Adam Bleckert; Huzefa Photowala; Simon Alford
Journal:  J Neurophysiol       Date:  2012-03-28       Impact factor: 2.714

4.  The effect of toxins on inorganic phosphate release during actin polymerization.

Authors:  Andrea Vig; Róbert Ohmacht; Eva Jámbor; Beáta Bugyi; Miklós Nyitrai; Gábor Hild
Journal:  Eur Biophys J       Date:  2011-01-04       Impact factor: 1.733

5.  Position and orientation of phalloidin in F-actin determined by X-ray fiber diffraction analysis.

Authors:  Toshiro Oda; Keiichi Namba; Yuichiro Maéda
Journal:  Biophys J       Date:  2005-01-14       Impact factor: 4.033

Review 6.  Fluorescent phallotoxins as probes for filamentous actin.

Authors:  H Faulstich; S Zobeley; G Rinnerthaler; J V Small
Journal:  J Muscle Res Cell Motil       Date:  1988-10       Impact factor: 2.698

7.  Arginylation regulates intracellular actin polymer level by modulating actin properties and binding of capping and severing proteins.

Authors:  Sougata Saha; Maureen M Mundia; Fangliang Zhang; Ryan W Demers; Farida Korobova; Tatyana Svitkina; Alex A Perieteanu; John F Dawson; Anna Kashina
Journal:  Mol Biol Cell       Date:  2010-02-24       Impact factor: 4.138

Review 8.  [50 years of phalloidine: its discovery, characterization and current and future applications in cell research].

Authors:  T Wieland
Journal:  Naturwissenschaften       Date:  1987-08

9.  Actin structure and function: roles in mitochondrial organization and morphogenesis in budding yeast and identification of the phalloidin-binding site.

Authors:  D G Drubin; H D Jones; K F Wertman
Journal:  Mol Biol Cell       Date:  1993-12       Impact factor: 4.138

10.  Participation of actin on Giardia lamblia growth and encystation.

Authors:  Araceli Castillo-Romero; Gloria Leon-Avila; Armando Perez Rangel; Rafael Cortes Zarate; Carlos Garcia Tovar; Jose Manuel Hernandez
Journal:  PLoS One       Date:  2009-09-23       Impact factor: 3.240
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.