Literature DB >> 406329

A solid phase fluorescent immunossay for the quantitation of the C4 component of human complement.

M W Burgett, S J Fairfield, J F Monthony.   

Abstract

A non-competitive method for the determination of the C4 component of human complement in serum is described. This procedure involves use of a specific antibody covalently attached to derivatized polyacrylamide beads and a fluorescently labeled specific antibody. Reproducible results were achieved for C4 in serum in the range of 10 mg/dl to 170 mg/dl within 2 h. C4 levels as low as 150 ng/ml can also be measured. Fluorescent immunoassay and radial immunodiffusion were used to determine C4 levels in healthy adults. Good agreement was found between the two methods.

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Year:  1977        PMID： 406329     DOI： 10.1016/0022-1759(77)90199-5

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  9 in total

1.  A solid phase fluorescent immunoassay for the rapid detection of virus antigen or antibodies in fetuses infected with porcine parvovirus.

Authors:  E Rivera; L Sjöland; K A Karlsson
Journal:  Arch Virol       Date:  1986       Impact factor: 2.574

2.  Comparison of colorimetric, fluorescent, and enzymatic amplification substrate systems in an enzyme immunoassay for detection of DNA-RNA hybrids.

Authors:  F Coutlee; R P Viscidi; R H Yolken
Journal:  J Clin Microbiol       Date:  1989-05       Impact factor: 5.948

3.  Characterization of antibody responses in legionellosis with an immunofluorometric assay.

Authors:  C M Black; L Pine; C B Reimer; R F Benson; T W Wells
Journal:  J Clin Microbiol       Date:  1982-06       Impact factor: 5.948

4.  Fluorescence immunoassay based on long time correlations of number fluctuations.

Authors:  D F Nicoli; J Briggs; V B Elings
Journal:  Proc Natl Acad Sci U S A       Date:  1980-08       Impact factor: 11.205

5.  Quantitative fluorescent immunoassay of antibodies to, and surface antigens of, Actinomyces viscosus.

Authors:  T P Gillis; J J Thompson
Journal:  J Clin Microbiol       Date:  1978-02       Impact factor: 5.948

6.  Detection and characterization of mouse monoclonal antibodies to epidemic typhus rickettsiae.

Authors:  C M Black; T Tzianabos; L F Roumillat; M A Redus; J E McDade; C B Reimer
Journal:  J Clin Microbiol       Date:  1983-09       Impact factor: 5.948

7.  Loss of transferrin receptor activity in Neisseria meningitidis correlates with inability to use transferrin as an iron source.

Authors:  J Tsai; D W Dyer; P F Sparling
Journal:  Infect Immun       Date:  1988-12       Impact factor: 3.441

8.  Oxidative radioiodination damage to human lactoferrin.

Authors:  A Rosenmund; C Kuyas; A Haeberli
Journal:  Biochem J       Date:  1986-11-15       Impact factor: 3.857

9.  Identification and characterization of receptors for granulocyte colony-stimulating factor on human placenta and trophoblastic cells.

Authors:  H Uzumaki; T Okabe; N Sasaki; K Hagiwara; F Takaku; M Tobita; K Yasukawa; S Ito; Y Umezawa
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205
  9 in total

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