Literature DB >> 4061808

Determination of nucleotide pools in plant tissue by high-performance liquid chromatography.

R Meyer, K G Wagner.   

Abstract

A procedure for the determination of nucleotide pools in plant tissue by HPLC is described. Sample preparation includes the extraction with 0.4 M HClO4, a purification step, which proved to be essential, on a disposable prepacked phenyl-bonded column, neutralization by KOH, and concentration by freeze-drying. The determination of a broad spectrum of ribonucleotides including the ribonucleosides was performed by combining anion-exchange and reversed-phase HPLC. Data are presented for suspension-cultured cells of Nicotiana tabacum and Datura innoxia and for the leaf and root of tobacco.

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Year:  1985        PMID： 4061808     DOI： 10.1016/0003-2697(85)90228-3

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  4 in total

1.  Uridine Diphosphate Glucose Metabolism and Callose Synthesis in Cultured Pollen Tubes of Nicotiana alata Link et Otto.

Authors:  H. Schlupmann; A. Bacic; S. M. Read
Journal:  Plant Physiol       Date:  1994-06       Impact factor: 8.340

2.  Nucleotide pools in suspension-cultured cells of Datura innoxia : I. Changes during growth of the batch culture.

Authors:  R Meyer; K G Wagner
Journal:  Planta       Date:  1985-12       Impact factor: 4.116

3.  Subcellular compartmentation of uridine nucleotides and nucleoside-5' -diphosphate kinase in leaves.

Authors:  J Dancer; H E Neuhaus; M Stitt
Journal:  Plant Physiol       Date:  1990-03       Impact factor: 8.340

4.  Enhanced nucleotide analysis enables the quantification of deoxynucleotides in plants and algae revealing connections between nucleoside and deoxynucleoside metabolism.

Authors:  Henryk Straube; Markus Niehaus; Sarah Zwittian; Claus-Peter Witte; Marco Herde
Journal:  Plant Cell       Date:  2021-04-17       Impact factor: 11.277
  4 in total

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