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Literature DB >> 405926

Improved procedure for crystallization of Clostridium botulinum type A toxic complexes.

Abstract

A modified protocol for the preparation of botulinum type A toxic crystals is decribed. The essential change was the substitution of chromatography on diethylaminoethyl-Sephadex for the step in which toxin was precipitated with ethanol. Colored materials and nucleic acids were removed by the chromatography so that crystal lots of greater uniformity could be produced. Significantly more of toxin in the starting culture was recovered as crystals. The lots of crystals obtained initially by either the original or the modified method contained an antigen that was eliminated when the lots were recrystallized.

Entities: Chemical Species

Mesh：

Substances：
Botulinum Toxins

Year: 1977 PMID： 405926 PMCID： PMC170797 DOI： 10.1128/aem.33.4.963-966.1977

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

9 in total

1. Botulinum Toxin (Type A); Including a Study of Shaking with Chloroform as a Step in the Isolation Procedure.

Authors: C Lamanna; H W Eklund; O E McElroy
Journal: J Bacteriol Date: 1946-07 Impact factor: 3.490

2. The lack of identity between hemagglutinin and the toxin of type A botulinal organism.

Authors: C LAMANNA; J P LOWENTHAL
Journal: J Bacteriol Date: 1951-06 Impact factor: 3.490

3. The Purification and Crystallization of Clostridium botulinum Type A Toxin.

Authors: C Lamanna; O E McElroy; H W Eklund
Journal: Science Date: 1946-05-17 Impact factor: 47.728

4. Disulfide-toxicity relationship of botulinal toxin types A, E, and F.

Authors: H Sugiyama; R Das Gupta; K H Yang
Journal: Proc Soc Exp Biol Med Date: 1973-07

5. Evaluation of type A botulinal toxin assays that use antitoxin to crystalline toxin.

Authors: H Sugiyama; I Oishi; B R Dasgupta
Journal: Appl Microbiol Date: 1974-02

6. The role of sulfhydryl groups in the activity of type A botulinum toxin.

Authors: J Knox; W P Brown; L Spero
Journal: Biochim Biophys Acta Date: 1970-08-21

7. Ultracentrifugal analysis of the crystalline toxin and isolated fractions of Clostridium botulinum type A.

Authors: D A Boroff; R Townend; U Fleck; B R DasGupta
Journal: J Biol Chem Date: 1966-11-25 Impact factor: 5.157

8. Statistical analysis of a rapid in vivo method for the titration of the toxin of Clostridium botulinum.

Authors: D A Boroff; U Fleck
Journal: J Bacteriol Date: 1966-11 Impact factor: 3.490

9. Molecular construction of Clostridium botulinum type A toxins.

Authors: S Sugii; G Sakaguchi
Journal: Infect Immun Date: 1975-12 Impact factor: 3.441

9 in total

10 in total

1. Botulinum toxin type A blocks the morphological changes induced by chemical stimulation on the presynaptic membrane of Torpedo synaptosomes.

Authors: J Marsal; G Egea; C Solsona; X Rabasseda; J Blasi
Journal: Proc Natl Acad Sci U S A Date: 1989-01 Impact factor: 11.205

2. Simplified purification method for Clostridium botulinum type E toxin.

Authors: J A Giménez; H Sugiyama
Journal: Appl Environ Microbiol Date: 1987-12 Impact factor: 4.792

Review 3. Clostridium botulinum neurotoxin.

Authors: H Sugiyama
Journal: Microbiol Rev Date: 1980-09

10. Noncorrelation between mouse toxicity and serologically assayed toxin in Clostridium botulinum type A culture fluids.

Authors: M J Betley; H Sugiyama
Journal: Appl Environ Microbiol Date: 1979-08 Impact factor: 4.792