Literature DB >> 4056409

A rapid and efficient immunization protocol for production of monoclonal antibodies reactive with autoantigens.

R Holmdahl, T Moran, M Andersson.   

Abstract

A simple, time-saving and efficient immunization method suitable for the production of mouse monoclonal antibody secreting hybridomas is described. Draining lymph nodes isolated 9 days after a primary immunization were used as the source of antibody producing cells. No systemic spread of antibody producing cells or specific antibodies could be detected. The present protocol was employed to generate a panel of collagen type II reactive monoclonal antibodies. Most of the monoclonals so generated were found to be of the IgG class.

Entities:  

Mesh：

Substances：

Year:  1985        PMID： 4056409     DOI： 10.1016/0022-1759(85)90260-1

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  19 in total

1.  Monoclonal Antibodies Recognizing Normal and Neoplastic Human Adrenal Cortex.

Authors:  Carin Backlin; Claes Juhlin; Lars Grimelius; Kristina Wiberg; Per Heilman; Goran Akerstrom; Jonas Rastad
Journal:  Endocr Pathol       Date:  1995       Impact factor: 3.943

2.  Loss of type IV collagen alpha 5 and alpha 6 chains in human invasive prostate carcinomas.

Authors:  P Dehan; D Waltregny; A Beschin; A Noel; V Castronovo; K Tryggvason; J De Leval; J M Foidart
Journal:  Am J Pathol       Date:  1997-10       Impact factor: 4.307

3.  T-cell receptor V beta haplotype and complement component C5 play no significant role for the resistance to collagen-induced arthritis in the SWR mouse.

Authors:  M Andersson; T J Goldschmidt; E Michaelsson; A Larsson; R Holmdahl
Journal:  Immunology       Date:  1991-06       Impact factor: 7.397

4.  Origin of the autoreactive anti-type II collagen response. I. Frequency of specific and multispecific B cells in primed murine lymph nodes.

Authors:  R Holmdahl; M Andersson; A Tarkowski
Journal:  Immunology       Date:  1987-07       Impact factor: 7.397

5.  Extra-articular cartilage affected in collagen-induced, but not pristane-induced, arthritis models.

Authors:  A-S Hansson; S Lu; R Holmdahl
Journal:  Clin Exp Immunol       Date:  2002-01       Impact factor: 4.330

6.  Dendritic cell-derived nitric oxide is involved in IL-4-induced suppression of experimental allergic encephalomyelitis (EAE) in Lewis rats.

Authors:  L Xu; Y Huang; J Yang; P H Van Der Meide; M Levi; B Wahren; H Link; B Xiao
Journal:  Clin Exp Immunol       Date:  1999-10       Impact factor: 4.330

7.  High-level production of alternatively spliced soluble interleukin-6 receptor in serum of patients with adult T-cell leukaemia/HTLV-I-associated myelopathy.

Authors:  S Horiuchi; W Ampofo; Y Koyanagi; A Yamashita; M Waki; A Matsumoto; M Yamamoto; N Yamamoto
Journal:  Immunology       Date:  1998-11       Impact factor: 7.397

8.  Monoclonal antibodies raised against mid-gut carcinoid tumor cells.

Authors:  C Juhlin; V Papanicolaou; K Backlin; L Klareskog; E Wilander; G Akerström; K Oberg; J Rastad
Journal:  World J Surg       Date:  1990 May-Jun       Impact factor: 3.352

9.  High antibody response to autologous type II collagen is restricted to H-2q.

Authors:  R Holmdahl; L Klareskog; M Andersson; C Hansen
Journal:  Immunogenetics       Date:  1986       Impact factor: 2.846

10.  High frequency of autoantibodies bearing cross-reactive idiotopes among hybridomas using VH7183 genes prepared from normal and autoimmune murine strains.

Authors:  B Bellon; A Manheimer-Lory; M Monestier; T Moran; A Dimitriu-Bona; F Alt; C Bona
Journal:  J Clin Invest       Date:  1987-04       Impact factor: 14.808
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.