Literature DB >> 405498

An analysis of the binding of fluorescence probes in mitochondrial systems.

W P Williams, D G Layton, C Johnston.   

Abstract

Measurements of the binding of the fluorescent probes 8-anilinonaphthalene-1-sulfonate (ANS) and ethidium ions to whole and disruped mitochondria and submitochondrial particles suggest that the inner mitochondrial membrane is freely permeable to the two probes. Equations relating the binding of permeant probes to the electro-chemical balance across the membrane of vesicular systems are derived and these equations used to analyze Scatchard plots of the binding of the two probes to energized and nonenergized mitochondria and EDTA particles.

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Year:  1977        PMID： 405498     DOI： 10.1007/bf01869510

Source DB:  PubMed          Journal:  J Membr Biol        ISSN： 0022-2631            Impact factor:   1.843


  20 in total

1.  Mitochondrial membrane potentials measured with microelectrodes: probable ionic basis.

Authors:  J T Tupper; H Tedeschi
Journal:  Science       Date:  1969-12-19       Impact factor: 47.728

2.  The influence of respiration and ATP hydrolysis on the proton-electrochemical gradient across the inner membrane of rat-liver mitochondria as determined by ion distribution.

Authors:  D G Nicholls
Journal:  Eur J Biochem       Date:  1974-12-16

3.  An analysis of the binding of 8-anilino-1-naphthalene-sulphonate to sub-mitochondrial particles.

Authors:  D G Layton; P Symmons; W P Williams
Journal:  FEBS Lett       Date:  1974-04-15       Impact factor: 4.124

4.  The influence of diffusion potentials across liposomal membranes on the fluorescence intensity of 1-anilinonaphthalene-8-sulphonate.

Authors:  E P Bakker; K van Dam
Journal:  Biochim Biophys Acta       Date:  1974-03-15

5.  Studies on the fluorescence and binding of 8-anilino-1-naphthalene sulfonate by submitochondrial particles.

Authors:  R A Harris
Journal:  Arch Biochem Biophys       Date:  1971-12       Impact factor: 4.013

6.  Anilinonaphthalenesulfonate fluorescence changes induced by non-emzymatic generation of membrane potential in mitochondria and submitochondrial particles.

Authors:  A A Jasaitis; V V Kuliene; V P Skulachev
Journal:  Biochim Biophys Acta       Date:  1971-04-06

7.  Estimation of membrane potential and pH difference across the cristae membrane of rat liver mitochondria.

Authors:  P Mitchell; J Moyle
Journal:  Eur J Biochem       Date:  1969-02

8.  Cooperative effects in binding by bovine serum albumin. I. The binding of 1-anilino-8-naphthalenesulfonate. Fluorimetric titrations.

Authors:  E Daniel; G Weber
Journal:  Biochemistry       Date:  1966-06       Impact factor: 3.162

9.  8-Anilinonaphthalene-1-sulphonate interaction with whole and disrupted mitochondria: a re-evaluation of the use of double-reciprocal plots in the derivation of binding parameters for fluorescent probes binding to mitochondrial membranes.

Authors:  N Gains; A P Dawson
Journal:  Biochem J       Date:  1975-04       Impact factor: 3.857

10.  On the nature of the energised state of submitochondrial particles; investigations with N-aryl naphthalene sulphonate probes.

Authors:  S J Ferguson; W J Lloyd; G K Radda
Journal:  Biochim Biophys Acta       Date:  1976-02-16
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  3 in total

1.  Membrane permeability transition promoted by phosphate enhances 1-anilino-8-naphthalene sulfonate fluorescence in calcium-loaded liver mitochondria.

Authors:  V T Maddaiah; U Kumbar
Journal:  J Bioenerg Biomembr       Date:  1993-08       Impact factor: 2.945

2.  Effect of chronic ethanol administration on energy metabolism and phospholipase A2 activity in rat liver.

Authors:  P I Spach; J W Parce; C C Cunningham
Journal:  Biochem J       Date:  1979-01-15       Impact factor: 3.857

3.  Characteristics of ethidium uptake by the trypanosomatid flagellates Crithidia fasciculata and Leptomonas seymouri.

Authors:  K P Coolbear; M Midgley
Journal:  Antimicrob Agents Chemother       Date:  1986-02       Impact factor: 5.191
  3 in total

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