Literature DB >> 4052813

Formulation of a novel synthetic medium for selectively culturing rat CNS neurons.

A Cestelli, G Savettieri, D Ferraro, F Vitale.   

Abstract

Dissociated cells from rat fetal cerebral hemispheres were grown in surface adhering culture using a novel synthetic medium (Maat medium) and compared with those grown either in the presence of serum or in the chemically defined medium described by Bottenstein and Sato. The addition of various compound combinations allowed us to lower insulin concentration to almost physiological levels. Maat medium improved the purity and longevity of neuronal cultures. The purity of neuronal cultures grown in different media was checked both by immunofluorescence and by the analysis of [3H]thymidine incorporation.

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Year:  1985        PMID： 4052813     DOI： 10.1016/0165-3806(85)90173-7

Source DB:  PubMed          Journal:  Brain Res        ISSN： 0006-8993            Impact factor:   3.252


  12 in total

1.  Accumulation of different c-erbA transcripts during rat brain development and in cortical neurons cultured in a synthetic medium.

Authors:  D Castiglia; A Cestelli; C Di Liegro; L Bonfanti; I Di Liegro
Journal:  Cell Mol Neurobiol       Date:  1992-06       Impact factor: 5.046

Review 2.  Neuronal cell cultures: a tool for investigations in developmental neurobiology.

Authors:  A Cestelli; G Savettieri; G Salemi; I Di Liegro
Journal:  Neurochem Res       Date:  1992-12       Impact factor: 3.996

3.  Binding of flunitrazepam to differentiating neurons cultured in a chemically defined, hormone-supplemented medium.

Authors:  G Savettieri; R Guarneri; G Salemi; V La Bella; D Ferraro; S Scondotto; F Piccoli
Journal:  Neurochem Res       Date:  1990-08       Impact factor: 3.996

4.  Triiodothyronine accelerates the synthesis of synapsin I in developing neurons from fetal rat brain cultured in a synthetic medium.

Authors:  G Salemi; D Ferraro; G Savettieri
Journal:  Neurochem Res       Date:  1990-08       Impact factor: 3.996

5.  Developmental changes of neuron-specific enolase mRNA in primary cultures of rat neurons.

Authors:  I Di Liegro; A Cestelli; G Barbieri; A Giallongo
Journal:  Cell Mol Neurobiol       Date:  1991-04       Impact factor: 5.046

6.  Effects of triiodothyronine (T3) on differentiation of rat cortical neurons in primary cultures.

Authors:  A Cestelli; D Castiglia; C Di Liegro; L Bonfanti; I Di Liegro
Journal:  Cytotechnology       Date:  1991-02       Impact factor: 2.058

7.  Posttranscriptional regulation of H1 zero and H3.3B histone genes in differentiating rat cortical neurons.

Authors:  M Scaturro; A Cestelli; D Castiglia; T Nastasi; I Di Liegro
Journal:  Neurochem Res       Date:  1995-08       Impact factor: 3.996

8.  Modulation of synapsin I gene expression in rat cortical neurons by extracellular matrix.

Authors:  G Savettieri; G A Mazzola; M B Rodriguez Sanchez; G Caruso; I Di Liegro; A Cestelli
Journal:  Cell Mol Neurobiol       Date:  1998-08       Impact factor: 5.046

9.  The dynamic properties of neuronal chromatin are modulated by triiodothyronine.

Authors:  A Cestelli; R Gristina; D Castiglia; C Di Liegro; G Savettieri; G Salemi; I Di Liegro
Journal:  Neurochem Res       Date:  1992-11       Impact factor: 3.996

10.  Expression of synapsin I gene in primary cultures of differentiating rat cortical neurons.

Authors:  I Di Liegro; G Savettieri; M Coppolino; M Scaturro; M Monte; T Nastasi; G Salemi; D Castiglia; A Cestelli
Journal:  Neurochem Res       Date:  1995-02       Impact factor: 3.996
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