Literature DB >> 4047281

Improved procedure for the purification of an iodinated protein: beta nerve growth factor.

D J Ennulat, R W Stach.   

Abstract

An improved procedure for the isolation of iodinated beta Nerve Growth Factor (125I-beta NGF) has been devised. Use of Centricon microconcentrators (Amicon) has allowed the facile and efficient recovery of ultrapure 125I-beta NGF in high yields. Centricon microconcentrators are supplied with two molecular weight cutoffs of 10 K and 30 K daltons. beta NGF is a basic protein with a molecular weight of 26 K daltons. It is therefore possible to filter the 125I-beta NGF through the 30 K filter (30 K Filtrate) leaving behind any aggregates or reactants greater than 30 K while the 125I-beta NGF can be retained and concentrated on the 10 K filter (10 K Retentate). Any free 125I is easily removed, passing through the 10 K filter and then being discarded. In this way 125I-beta NGF can be easily purified.

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Year:  1985        PMID： 4047281     DOI： 10.1007/BF00964636

Source DB:  PubMed          Journal:  Neurochem Res        ISSN： 0364-3190            Impact factor:   3.996


  9 in total

1.  A more rapid method for the isolation of the 7S nerve growth factor complex.

Authors:  R W Stach; B J Wagner; B M Stach
Journal:  Anal Biochem       Date:  1977-11       Impact factor: 3.365

2.  Properties of the specific binding of 125I-nerve growth factor to responsive peripheral neurons.

Authors:  W A Frazier; L F Boyd; R A Bradshaw
Journal:  J Biol Chem       Date:  1974-09-10       Impact factor: 5.157

3.  Interaction of [125I] beta nerve growth factor with acidic proteins.

Authors:  K W Rusenko; R W Stach
Journal:  Neurochem Res       Date:  1981-03       Impact factor: 3.996

4.  Sequestration of 125I-labeled beta nerve growth factor by sympathetic neurons.

Authors:  E J Olender; R W Stach
Journal:  J Biol Chem       Date:  1980-10-10       Impact factor: 5.157

Review 5.  Nerve growth factor.

Authors:  R A Bradshaw
Journal:  Annu Rev Biochem       Date:  1978       Impact factor: 23.643

6.  Nerve growth factor receptors. Characterization of two distinct classes of binding sites on chick embryo sensory ganglia cells.

Authors:  A Sutter; R J Riopelle; R M Harris-Warrick; E M Shooter
Journal:  J Biol Chem       Date:  1979-07-10       Impact factor: 5.157

7.  Binding of nerve growth factor receptor in sympathetic ganglia.

Authors:  S P Banerjee; S H Snyder; P Cuatrecasas; L A Greene
Journal:  Proc Natl Acad Sci U S A       Date:  1973-09       Impact factor: 11.205

8.  Properties of the beta nerve growth factor receptor of avian dorsal root ganglia.

Authors:  K Herrup; E M Shooter
Journal:  Proc Natl Acad Sci U S A       Date:  1973-12       Impact factor: 11.205

9.  Covalent attachment of 125I-beta nerve growth factor to its receptors on sympathetic neurons.

Authors:  R W Stach; K W Rusenko
Journal:  J Neurosci Res       Date:  1984       Impact factor: 4.164
  9 in total
  1 in total

1.  Induction of the high-affinity nerve growth factor receptor on embryonic chicken sensory nerve cells by elevated potassium.

Authors:  D J Ennulat; R W Stach
Journal:  Neurochem Res       Date:  1987-10       Impact factor: 3.996
  1 in total

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