| Literature DB >> 4046600 |
M Pons, T E Robinson, L Mercier, E B Thompson, S S Simons.
Abstract
An intensely fluorescent rhodamine derivative of dexamethasone (i.e. Dex-C2-Rho) was synthesized. Dex-C2-Rho possessed high affinity for HTC cell glucocorticoid receptors in cell-free systems. Whole cell activity and receptor affinity of Dex-C2-Rho were both much lower, apparently due to problems with cell permeability and/or metabolism. A specific, fluorescent receptor-steroid complex at concentrations as low as 1 X 10(-9) M could readily be observed with crude HTC cell receptors after removal of the free Dex-C2-Rho. This appears to be the first report of a fluorescent glucocorticoid receptor-steroid complex.Entities:
Mesh：
Substances：
Year: 1985 PMID： 4046600 DOI： 10.1016/0022-4731(85)90404-2
Source DB: PubMed Journal: J Steroid Biochem ISSN： 0022-4731 Impact factor: 4.292