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Literature DB >> 404521

Buoyant density fractionation of Drosophila melanogaster chromatin.

Abstract

Buoyant density fractionation of Drosophila melanogaster chromatin utilizing low molecular weight molecules such as actinomycin-D to induce changes in buoyant density has been investigated. Fractions of chromatin containing identifiable repeated DNA sequences could be isolated using actinomycin-D as the selective agent. Protein displacement from the chromatin complex was found to be a prerequisite for the observed buoyant density changes.

Entities: Chemical Species

Mesh：

Substances：
Chromatin
Dactinomycin
DNA

Year: 1977 PMID： 404521 DOI： 10.1007/bf00643476

Source DB: PubMed Journal: Mol Biol Rep ISSN： 0301-4851 Impact factor: 2.316

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References

13 in total

1. Intercalating agents as probes of the spatial relationship between chromatin components.

Authors: C S Baxter; P Byvoet
Journal: Biochem Biophys Res Commun Date: 1975-03-03 Impact factor: 3.575

2. Buoyant density sedimentation of macromolecules in sodium iothalamate density gradients.

Authors: P Serwer
Journal: J Mol Biol Date: 1975-03-05 Impact factor: 5.469

3. Fractionation of unfixed chromatin by buoyant-density centrifugation.

Authors: D Rickwood; A Hell; S Malcolm; G D Birnie; A J MacGillivray; J Paul
Journal: Biochim Biophys Acta Date: 1974-07-11

4. Buoyant densities and hydration of nucleic acids, proteins and nucleoprotein complexes in metrizamide.

Authors: G D Birnie; D Rickwood; A Hell
Journal: Biochim Biophys Acta Date: 1973-12-07

5. Theoretical aspects of DNA-protein interactions: co-operative and non-co-operative binding of large ligands to a one-dimensional homogeneous lattice.

Authors: J D McGhee; P H von Hippel
Journal: J Mol Biol Date: 1974-06-25 Impact factor: 5.469

Buoyant density fractionation of Drosophila melanogaster chromatin.

1. Intercalating agents as probes of the spatial relationship between chromatin components.

2. Buoyant density sedimentation of macromolecules in sodium iothalamate density gradients.

3. Fractionation of unfixed chromatin by buoyant-density centrifugation.

4. Buoyant densities and hydration of nucleic acids, proteins and nucleoprotein complexes in metrizamide.

5. Theoretical aspects of DNA-protein interactions: co-operative and non-co-operative binding of large ligands to a one-dimensional homogeneous lattice.

6. Interphase chromosomal deoxyribonucleoprotein isolated as a discrete structure from cultured cells.

7. Binding of actinomycin D to calf thymus chromatin.

8. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

9. Protein content of chromatin fractions separated by sucrose gradient centrifugation.

10. Isopycnic centrifugation of chromatin in renografin solutions.