Literature DB >> 4044310

Cytochemical localization of alkaline phosphatase in the cell membrane of Dictyostelium discoideum amebae.

I Glomp, D Schäfer, B Hess.   

Abstract

We demonstrated that alkaline phosphatase was localized on the cell membrane of Dictyostelium discoideum amebae and on isolated plasma membranes. The enzyme activity was specifically inhibited by 0.01 M KCN or cysteine. The same method could also be applied to baker's yeast and MDCK cells (dog kidney cells in vitro).

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Year:  1985        PMID： 4044310     DOI： 10.1007/BF00953993

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  12 in total

1.  Studies on the alkaline phosphatase and 5'-nucleotidase of Dictyostelium discoideum.

Authors:  A Lee; K Chance; C Weeks; G Weeks
Journal:  Arch Biochem Biophys       Date:  1975-12       Impact factor: 4.013

2.  Embedding in epoxy resins for ultrathin sectioning in electron microscopy.

Authors:  K C RICHARDSON; L JARETT; E H FINKE
Journal:  Stain Technol       Date:  1960-11

3.  [Effects of 8 various aldehydes and of the pH value on the localization of glucose-6-phosphatase in cultured cells].

Authors:  D Schäfer; M Hündgen
Journal:  Histochemie       Date:  1971

4.  Fine structural localization of acid and alkaline phosphatase activities in the absorbing cells of the duodenum of rodents.

Authors:  J Hugon; M Borgers
Journal:  Histochemie       Date:  1968

5.  Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture.

Authors:  D J Watts; J M Ashworth
Journal:  Biochem J       Date:  1970-09       Impact factor: 3.857

6.  The purification and characterization of Dictyostelium discoideum plasma membranes.

Authors:  N R Gilkes; G Weeks
Journal:  Biochim Biophys Acta       Date:  1977-01-04

7.  A direct lead method for the electron microscopic visualization of alkaline phosphatase activity.

Authors:  J Hugon; M Borgers
Journal:  J Histochem Cytochem       Date:  1966-05       Impact factor: 2.479

8.  The isolation and subfractionation of plasma membrane from the cellular slime mould Dictyostelium discoideum.

Authors:  A A Green; P C Newell
Journal:  Biochem J       Date:  1974-05       Impact factor: 3.857

9.  Improvements in epoxy resin embedding methods.

Authors:  J H LUFT
Journal:  J Biophys Biochem Cytol       Date:  1961-02

Review 10.  Plasma membranes of mammalian cells: a review of methods for their characterization and isolation.

Authors:  J W DePierre; M L Karnovsky
Journal:  J Cell Biol       Date:  1973-02       Impact factor: 10.539
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  2 in total

1.  Isolation and characterization of a plasma membrane calcium pump from Dictyostelium discoideum.

Authors:  J L Milne; M B Coukell
Journal:  Biochem J       Date:  1988-01-01       Impact factor: 3.857

2.  Cell fractionation, detergent sensitivity and solubilization of Dictyostelium adenylate cyclase and guanylate cyclase.

Authors:  P M Janssens; H W van Essen; J J Guijt; A de Waal; R van Driel
Journal:  Mol Cell Biochem       Date:  1987-07       Impact factor: 3.396
  2 in total

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