| Literature DB >> 4044 |
C G Friedrich, B Friedrich, H G Schlegel.
Abstract
Properties and regulation of anthranilate synthase from Alcaligenes eutrophus H 16 were investigated. Anthranilate synthase was partially purified from crude extracts by affinity chromatography on tryptophan-substituted Sepharose, and was used for kinetic measurements. During the purification procedure the enzyme was stabilized by 50 mM L-glutamine or during chromatography on DEAE- cellulose and Sephadex G-200 with 30% glucerol, respectively.Entities:
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Year: 1976 PMID: 4044 DOI: 10.1007/BF00446831
Source DB: PubMed Journal: Arch Microbiol ISSN: 0302-8933 Impact factor: 2.552