Literature DB >> 4042112

A convenient method for methylation of glycoprotein glycans in small amounts by using lithium methylsulfinyl carbanion.

J P Parente, P Cardon, Y Leroy, J Montreuil, B Fournet, G Ricart.   

Abstract

Treatment of dimethyl sulfoxide with butyllithium leads to rapid formation of lithium methylsulfinyl carbanion. The reaction products tend to be significantly freer from impurities when lithium methylsulfinyl carbanion is used rather than sodium or potassium methylsulfinyl carbanion. This reagent gives less background in g.l.c. and thus may be used to methylate micro-quantities of glycoprotein glycans (down to 10 micrograms) without the necessity of identifying methyl ethers by mass spectrometry.

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Year:  1985        PMID： 4042112     DOI： 10.1016/s0008-6215(00)90753-5

Source DB:  PubMed          Journal:  Carbohydr Res        ISSN： 0008-6215            Impact factor:   2.104


  26 in total

1.  Waxy Chlamydomonas reinhardtii: monocellular algal mutants defective in amylose biosynthesis and granule-bound starch synthase activity accumulate a structurally modified amylopectin.

Authors:  B Delrue; T Fontaine; F Routier; A Decq; J M Wieruszeski; N Van Den Koornhuyse; M L Maddelein; B Fournet; S Ball
Journal:  J Bacteriol       Date:  1992-06       Impact factor: 3.490

2.  Osmoregulated periplasmic glucans of Erwinia chrysanthemi.

Authors:  V Cogez; P Talaga; J Lemoine; J P Bohin
Journal:  J Bacteriol       Date:  2001-05       Impact factor: 3.490

3.  Structures of the alpha(1-3)-galactose-containing asparagine-linked glycans of a Lewis lung carcinoma cell subline resistant to Aleuria aurantia agglutinin: elucidation by 1H-NMR spectroscopy.

Authors:  H Debray; D Dus; J M Wieruszeski; G Strecker; J Montreuil
Journal:  Glycoconj J       Date:  1991-02       Impact factor: 2.916

4.  Galactosylated fucose epitopes in nematodes: increased expression in a Caenorhabditis mutant associated with altered lectin sensitivity and occurrence in parasitic species.

Authors:  Shi Yan; Silvia Bleuler-Martinez; David Fernando Plaza; Markus Künzler; Markus Aebi; Anja Joachim; Ebrahim Razzazi-Fazeli; Verena Jantsch; Rudolf Geyer; Iain B H Wilson; Katharina Paschinger
Journal:  J Biol Chem       Date:  2012-06-25       Impact factor: 5.157

5.  Structural elucidation of zwitterionic carbohydrates derived from glycosphingolipids of the porcine parasitic nematode Ascaris suum.

Authors:  Claudia H Friedl; Günter Lochnit; Ulrich Zähringer; Ute Bahr; Rudolf Geyer
Journal:  Biochem J       Date:  2003-01-01       Impact factor: 3.857

6.  Chemical and immunological characterization of the extracellular galactomannan of Aspergillus fumigatus.

Authors:  J P Latgé; H Kobayashi; J P Debeaupuis; M Diaquin; J Sarfati; J M Wieruszeski; E Parra; J P Bouchara; B Fournet
Journal:  Infect Immun       Date:  1994-12       Impact factor: 3.441

7.  Periplasmic glucans of Pseudomonas syringae pv. syringae.

Authors:  P Talaga; B Fournet; J P Bohin
Journal:  J Bacteriol       Date:  1994-11       Impact factor: 3.490

8.  Storage, Photosynthesis, and Growth: The Conditional Nature of Mutations Affecting Starch Synthesis and Structure in Chlamydomonas.

Authors:  N. Libessart; M. L. Maddelein; NVd. Koornhuyse; A. Decq; B. Delrue; G. Mouille; C. D'Hulst; S. Ball
Journal:  Plant Cell       Date:  1995-08       Impact factor: 11.277

9.  Structural analysis of novel rhamnose-branched oligosaccharides from the glycophosphosphingolipids of Leptomonas samueli.

Authors:  J O Previato; R Wait; C Jones; L Mendonça-Previato
Journal:  Glycoconj J       Date:  1994-02       Impact factor: 2.916

10.  Cell wall beta-(1,6)-glucan of Saccharomyces cerevisiae: structural characterization and in situ synthesis.

Authors:  Vishukumar Aimanianda; Cécile Clavaud; Catherine Simenel; Thierry Fontaine; Muriel Delepierre; Jean-Paul Latgé
Journal:  J Biol Chem       Date:  2009-03-11       Impact factor: 5.157
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