Literature DB >> 4041144

Biological and immunological characteristics of 125I-4Tyr and -18Tyr Escherichia coli heat-stable enterotoxin species purified by high-performance liquid chromatography.

M R Thompson, M Luttrell, G Overmann, R A Giannella.   

Abstract

A rapid procedure for the separation of Escherichia coli heat-stable enterotoxin iodination products is described. A two-step procedure using reverse-phase chromatography results in the separation of native, noniodinated heat-stable enterotoxins from several distinctly iodinated species which differ in their ability to bind to brush border receptors. Sequence analysis of the radioligands demonstrated mutually exclusive labeling at the 4Tyr and 18Tyr residues. These tracers have been immunologically and biologically characterized and have been shown to be antigenically similar but to differ in their toxicity for suckling mice. The radioligands separated by this procedure demonstrate stable behavior for a period of months.

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Year:  1985        PMID： 4041144     DOI： 10.1016/0003-2697(85)90623-2

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  13 in total

1.  Nucleotide regulation of heat-stable enterotoxin receptor binding and of guanylate cyclase activation.

Authors:  L C Katwa; C D Parker; J K Dybing; A A White
Journal:  Biochem J       Date:  1992-05-01       Impact factor: 3.857

2.  Identification of ligand recognition sites in heat-stable enterotoxin receptor, membrane-associated guanylyl cyclase C by site-directed mutational analysis.

Authors:  A Wada; T Hirayama; H Kitaura; J Fujisawa; M Hasegawa; Y Hidaka; Y Shimonishi
Journal:  Infect Immun       Date:  1996-12       Impact factor: 3.441

3.  Citrobacter freundii produces an 18-amino-acid heat-stable enterotoxin identical to the 18-amino-acid Escherichia coli heat-stable enterotoxin (ST Ia).

Authors:  A Guarino; R Giannella; M R Thompson
Journal:  Infect Immun       Date:  1989-02       Impact factor: 3.441

4.  Association and dissociation of Escherichia coli heat-stable enterotoxin from rat brush border membrane receptors.

Authors:  M B Cohen; M R Thompson; G J Overmann; R A Giannella
Journal:  Infect Immun       Date:  1987-02       Impact factor: 3.441

5.  Binding of E. coli heat-stable enterotoxin to rat intestinal brush borders and to basolateral membranes.

Authors:  A Guarino; M B Cohen; G Overmann; M R Thompson; R A Giannella
Journal:  Dig Dis Sci       Date:  1987-09       Impact factor: 3.199

6.  Guanylyl cyclase C is a selective marker for metastatic colorectal tumors in human extraintestinal tissues.

Authors:  S L Carrithers; M T Barber; S Biswas; S J Parkinson; P K Park; S D Goldstein; S A Waldman
Journal:  Proc Natl Acad Sci U S A       Date:  1996-12-10       Impact factor: 11.205

7.  Age-dependent changes in affinity-labeled receptors for Escherichia coli heat-stable enterotoxin in the swine intestine.

Authors:  L C Katwa; C D Parker; A A White
Journal:  Infect Immun       Date:  1991-12       Impact factor: 3.441

8.  Activation of intestinal brush border guanylate cyclase by aromatic disulphide compounds.

Authors:  M M elDeib; C D Parker; A A White
Journal:  Biochem J       Date:  1991-04-01       Impact factor: 3.857

9.  Blinded, two-laboratory comparative analysis of Escherichia coli heat-stable enterotoxin production by using monoclonal antibody enzyme-linked immunosorbent assay, radioimmunoassay, suckling mouse assay, and gene probes.

Authors:  M R Thompson; R L Jordan; M A Luttrell; H Brandwein; J B Kaper; M M Levine; R A Giannella
Journal:  J Clin Microbiol       Date:  1986-11       Impact factor: 5.948

10.  Presence of functional receptors for the Escherichia coli heat-stable enterotoxin in the gastrointestinal tract of the chicken.

Authors:  L C Katwa; A A White
Journal:  Infect Immun       Date:  1992-09       Impact factor: 3.441
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