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Literature DB >> 4041006

Crosslinking of chromosomal antigen common in human tumors to DNA by cis-diamminedichloroplatinum (II).

Abstract

The antisera specific for dehistonized HeLa cell chromatin were obtained by injecting rabbits or goats. Treatment of chromatin with cis-DDP crosslinked the active proteins to DNA thus preventing dissociation of the proteins in a high salt environment. Immunochemical staining of electrophoretically separated chromosomal proteins transferred to nitrocellulose sheets revealed that cis-DDP among others crosslinked the protein with m.w. of about 81 000. This protein is the only major protein antigen presented in several human tumors and absent in normal human tissues.

Entities: Chemical Disease Gene Species

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Year: 1985 PMID： 4041006 DOI： 10.1007/bf00778527

Source DB: PubMed Journal: Mol Biol Rep ISSN： 0301-4851 Impact factor: 2.316

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References

15 in total

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Authors: E WASSERMAN; L LEVINE
Journal: J Immunol Date: 1961-09 Impact factor: 5.422

2. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors: H Towbin; T Staehelin; J Gordon
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

Crosslinking of chromosomal antigen common in human tumors to DNA by cis-diamminedichloroplatinum (II).

1. Quantitative micro-complement fixation and its use in the study of antigenic structure by specific antigen-antibody inhibition.

2. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

3. The mechanism of action of antitumor platinum compounds.

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

5. Proteins of chromatin in template restriction. I. RNA synthesis in vitro.

6. Proteins of chromatin in template restriction. 3. The macromolecules in specific restriction of the chromatin DNA.

7. Gamma-radiation-induced crosslinking of cell-specific chromosomal nonhistone protein-DNA complexes in HeLa chromatin.

8. Identification of tissue-specific nuclear antigens transferred to nitrocellulose from polyacrylamide gels.

9. Cross-linking of chromosomal non-histone proteins to DNA by UV radiation and some antitumor drugs.

10. Effects of cis-diamminedichloroplatinum (CDDP) on HeLa cell non-histone nuclear proteins.