Reconciliation of the absorption change at 325 nm and other flash-yield determinations of concentrations of active photosystem II centers.

The concentration of photosystem II was determined in thylakoids of dwarf peas by the use of the following methods: absorption change at 325 nm; atrazine binding; and flash yields of oxygen evolution (Emerson-Arnold method), of protons from oxidation of water, and of reduction of DCIP. For the first time all of the flash-yield measurements have been done on the same sample and give equivalent values for the concentration of photosystem II. Agreement of the absorption change measurement at 325 nm with the other measurements was accomplished by the introduction of important improvements to the methods of Melis and co-workers [Proc. Natl. Acad. Sci. USA (1980) 77, 4712-4716]. The atrazine-binding method gave photosystem II values that were twice as large as any of the other photosystem II measurements. Possible reasons are discussed for this discrepancy in terms of the secondary acceptor (Q400) of Ikegami and Katoh [Plant Cell Physiol. (1973) 14, 829-836]. The concentration of photosystem I was measured by absorption change at 705 nm. From the concentration values of photosystem II and I the system II/I stoichiometry was calculated.