Literature DB >> 4037668

Continuous-flow assay for urinary oxalate using immobilised oxalate oxidase.

G P Kasidas, G A Rose.   

Abstract

A continuous-flow assay for measuring oxalate in urine is described. Covalently attached oxalate oxidase (EC 1.2.3.4) is used to oxidize the oxalate anion to carbon dioxide and hydrogen peroxide. The formed hydrogen peroxide is measured colorimetrically (A580) with an established reaction using horseradish peroxidase (EC 1.11.17), 3-methyl-2-benzothiazolinone hydrazone (MBTH) and 3-dimethylaminobenzoic acid (DMAB). Ascorbate interference is eliminated by treating the urine sample with sodium nitrite prior to assaying. The assay is accurate (mean recovery of added oxalate in spiked urine sample is 93 +/- 11%), sensitive (detection limit 1.0 mumol/L), reproducible (within-batch CV 3.5%; between-batch CV 5%) and relatively rapid (15 samples/h). This assay correlates well (R = 0.99) with another established enzymatic method (using oxalate decarboxylase).

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Year:  1985        PMID： 4037668     DOI： 10.1177/000456328502200415

Source DB:  PubMed          Journal:  Ann Clin Biochem        ISSN： 0004-5632            Impact factor:   2.057


  10 in total

1.  Urinary oxalate and glycolate excretion and plasma oxalate concentration.

Authors:  T M Barratt; G P Kasidas; I Murdoch; G A Rose
Journal:  Arch Dis Child       Date:  1991-04       Impact factor: 3.791

2.  Effects of oral pyridoxine upon plasma and 24-hour urinary oxalate levels in normal subjects and stone formers with idiopathic hypercalciuria.

Authors:  P Edwards; S Nemat; G A Rose
Journal:  Urol Res       Date:  1990

3.  Oxalobacter formigenes-Derived Bioactive Factors Stimulate Oxalate Transport by Intestinal Epithelial Cells.

Authors:  Donna Arvans; Yong-Chul Jung; Dionysios Antonopoulos; Jason Koval; Ignacio Granja; Mohamed Bashir; Eltayeb Karrar; Jayanta Roy-Chowdhury; Mark Musch; John Asplin; Eugene Chang; Hatim Hassan
Journal:  J Am Soc Nephrol       Date:  2016-10-13       Impact factor: 10.121

4.  Pattern of urolithiasis in a general hospital. A prospective study.

Authors:  M S Khalifa; A al Shazly; P C Reavey
Journal:  Int Urol Nephrol       Date:  1989       Impact factor: 2.370

Review 5.  Urinary chemistry of the normal Sprague-Dawley rat.

Authors:  P N Shevock; S R Khan; R L Hackett
Journal:  Urol Res       Date:  1993

6.  [Type I oxalosis in childhood--studies within the scope of terminal renal failure in the child].

Authors:  M Frosch; E Kuwertz-Bröking; M Bulla; D B von Bassewitz; D B Leusmann
Journal:  Klin Wochenschr       Date:  1989-11-17

7.  Idiopathic hypercalciuria. Urate and other ions in urine before and on various long term treatments.

Authors:  H S Gill; G A Rose
Journal:  Urol Res       Date:  1985

8.  Enhanced gastrointestinal passive paracellular permeability contributes to the obesity-associated hyperoxaluria.

Authors:  Mohamed Bashir; Jon Meddings; Altayeb Alshaikh; Daniel Jung; Kim Le; Ruhul Amin; Sireesha Ratakonda; Sapna Sharma; Ignacio Granja; Mustafa Satti; John Asplin; Hatim Hassan
Journal:  Am J Physiol Gastrointest Liver Physiol       Date:  2018-10-11       Impact factor: 4.052

9.  Urinary calcium and oxalate excretion in children.

Authors:  G S Reusz; M Dobos; D Byrd; P Sallay; M Miltényi; T Tulassay
Journal:  Pediatr Nephrol       Date:  1995-02       Impact factor: 3.714

10.  A randomised Phase II/III study to evaluate the efficacy and safety of orally administered Oxalobacter formigenes to treat primary hyperoxaluria.

Authors:  Dawn Milliner; Bernd Hoppe; Jaap Groothoff
Journal:  Urolithiasis       Date:  2017-07-17       Impact factor: 3.436
  10 in total

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