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Literature DB >> 4033872

Isolation and purification of myelin proteolipid protein using high speed gel filtration in sodium dodecyl sulfate.

Abstract

Small and preparative gel filtration columns were studied for high pressure liquid chromatography of myelin proteins in sodium dodecyl sulfate. The preparative column proved useful for isolating and purifying proteolipid protein almost free (0.3-0.5%) of myelin basic protein as demonstrated by SDS-PAGE, MBP RIA, and immunoblotting. The small columns were not as useful as SDS-PAGE for analytical purposes.

Entities: Chemical Disease Gene

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Year: 1985 PMID： 4033872 DOI： 10.1007/bf00964543

Source DB: PubMed Journal: Neurochem Res ISSN： 0364-3190 Impact factor: 3.996

9 in total

1. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

2. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors: H Towbin; T Staehelin; J Gordon
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

3. Myelin proteins from different regions of the central nervous system.

Authors: M B Lees; S A Paxman
Journal: J Neurochem Date: 1974-10 Impact factor: 5.372

4. Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system.

Authors: D M Neville
Journal: J Biol Chem Date: 1971-10-25 Impact factor: 5.157

5. Structure of the proteolipid protein extracted from bovine central nervous system myelin with nondenaturing detergents.

Authors: R Smith; J Cook; P A Dickens
Journal: J Neurochem Date: 1984-02 Impact factor: 5.372

1 in total

1. Long-term inhibition of murine experimental autoimmune encephalomyelitis using CTLA-4-Fc supports a key role for CD28 costimulation.

Authors: A H Cross; T J Girard; K S Giacoletto; R J Evans; R M Keeling; R F Lin; J L Trotter; R W Karr
Journal: J Clin Invest Date: 1995-06 Impact factor: 14.808

1 in total

Isolation and purification of myelin proteolipid protein using high speed gel filtration in sodium dodecyl sulfate.

1. Protein measurement with the Folin phenol reagent.

2. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

3. Myelin proteins from different regions of the central nervous system.

4. Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system.

5. Structure of the proteolipid protein extracted from bovine central nervous system myelin with nondenaturing detergents.

6. Purification and immunohistochemical localization of rat brain myelin proteolipid protein.

7. A radioimmunoassay for myelin basic protein and its use for quantitative measurements.

8. Radioimmunoassay for central nervous system myelin-specific proteolipid protein.

9. High-speed gel filtration of polypeptides in sodium dodecyl sulfate.

1. Long-term inhibition of murine experimental autoimmune encephalomyelitis using CTLA-4-Fc supports a key role for CD28 costimulation.