Ontogenic variations in the interactions of lactate dehydrogenase isozymes with cellular structure.

The interactions of the LDH isozymes with cellular structure have been studied in the major tissues of the mouse during development. Overall, there is a clear indication that interactions between LDH and cellular structure are appreciable in all major tissues and at all stages of development, and an analysis of the isozyme status of the enzyme in both soluble and bound compartments has been effected. Information has been provided supporting the preferential binding of the A type activity to actin filaments. The data provided also support the concept of pelletable B type LDH in kidney and brain during the postnatal period. Binding was particularly extensive in the early embryonic stages, and the significance of this phenomenon, and the subcellular interactions observed to varying degrees in all the tissues throughout development, have been discussed in relation to the known metabolic characteristics of these separate tissue situations.