| Literature DB >> 4031358 |
G Lefèvre, F Tallet, S Baassou, J Agneray, J Yonger, D Raichvarg.
Abstract
A fast and practicable gas-liquid chromatographic method for the quantitation of non-esterified fatty acids (C14:0-C18:2) from 100 microliter plasma is described. This technique includes extraction, purification using the solvents n-heptane and 0.5 M Na2CO3, and methylation by 0.1 M HCl-methanol. Extraction, quantification and optimal methylation conditions without transmethylation have been investigated. Reproducibility is good and results agree with values found in the literature.Entities:
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Year: 1985 PMID： 4031358 DOI： 10.1016/0165-022x(85)90048-x
Source DB: PubMed Journal: J Biochem Biophys Methods ISSN： 0165-022X