Sensitive liquid chromatographic method for physostigmine in biological fluids using dual-electrode electrochemical detection.

A liquid chromatographic method using dual-electrode detection has been developed for determination of physostigmine in biological fluids. The limit of detection is in the order of 25-50 pg mol-1 of plasma. A high sample throughput is obtained by a single solvent extraction step and autoinjection into the chromatograph. Data following oral doses of physostigmine are presented.