The effect of diltiazem on noradrenaline release.

The effects of diltiazem in rat tail arteries and guinea-pig vasa deferentia have been investigated. Superfusion of the rat tail artery with diltiazem (10(-6) - 10(-4) M) resulted in a dose-related increase in 3H-overflow (P less than 0.001) both in Wistar Kyoto (WKY) and in spontaneously hypertensive (SHR) rats. Release of 3H by transmural stimulation (1 Hz, 2 ms, 10 V) was also much greater in vessels perfused with diltiazem; this effect was dose-dependent. Diltiazem did not significantly alter the proportion of noradrenaline and its metabolites in 3H-overflow, as analysed by column chromatography. In the vasa deferentia of guinea-pigs, diltiazem (10(-9) - 10(-5) M) increased spontaneous 3H-release. The results indicate that diltiazem acts on sympathetic nerves and causes the release of noradrenaline.