Literature DB >> 4026821

Isolation and partial characterization of a novel basement membrane collagen.

R Dixit, M W Harrison, S N Dixit.   

Abstract

A guanidine-HCl extraction of lens capsule basement membrane dissolves collagenous material. This material was fractionated on an Agarose A-5M column. Fractions 1, 2 and 3 were further purified and partially characterized immunochemically and by amino acid analysis. Fraction 3 has a molecular weight of 55,000 when compared with collagen type I standard. The CNBr peptide pattern and composition of fraction 3 are different from those of alpha 1 (IV) 95K and alpha 2 (IV) 95K chains. The results described suggest the presence of a new chain in lens capsule basement membrane.

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Year:  1985        PMID： 4026821     DOI： 10.1016/0006-291x(85)90373-0

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  5 in total

1.  Fundamental study on the mechanism of DNA degradation in tissues fixed in formaldehyde.

Authors:  Y Tokuda; T Nakamura; K Satonaka; S Maeda; K Doi; S Baba; T Sugiyama
Journal:  J Clin Pathol       Date:  1990-09       Impact factor: 3.411

2.  Tissue extraction of DNA and RNA and analysis by the polymerase chain reaction.

Authors:  D P Jackson; F A Lewis; G R Taylor; A W Boylston; P Quirke
Journal:  J Clin Pathol       Date:  1990-06       Impact factor: 3.411

3.  Undifferentiated F9 embryonal carcinoma cells produce a short-chain collagen molecule.

Authors:  B D Smith; C T Baldwin
Journal:  Biochem J       Date:  1988-10-01       Impact factor: 3.857

4.  Partial characterization of a low molecular weight human collagen that undergoes alternative splicing.

Authors:  T Pihlajaniemi; R Myllylä; J Seyer; M Kurkinen; D J Prockop
Journal:  Proc Natl Acad Sci U S A       Date:  1987-02       Impact factor: 11.205

5.  An immunohistochemical and electron microscopic study of extra-renal basement membranes in dogs with Samoyed hereditary glomerulopathy.

Authors:  P S Thorner; B Jansen; R Baumal; R V Harrison; R J Mount; V E Valli; P M Spicer; P M Marrano
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1988
  5 in total

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