Literature DB >> 4025820

Method for extraction of proteins from green plant tissues for two-dimensional polyacrylamide gel electrophoresis.

F Cremer, C Van de Walle.   

Abstract

A method has been developed for the extraction of proteins from green plant tissues to be used in two-dimensional polyacrylamide gel electrophoresis. Three purification steps were necessary to overcome the problems due to streaking, charge heterogeneity, and other artifacts: After it was ground in liquid nitrogen, the powdered material was stirred in the presence of insoluble polyvinylpyrrolidone for binding phenols, and sodium ascorbate for binding quinones; proteins were precipitated with ammonium sulfate, and the sample was dialyzed. Hundreds of proteins could be detected after Coomassie blue staining using 200 micrograms of proteins from apical buds of Sinapis alba L.

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Year:  1985        PMID： 4025820     DOI： 10.1016/0003-2697(85)90004-1

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  7 in total

1.  Diurnal Rhythmicity in the Pattern of mRNAs in the Leaves of Sinapis alba.

Authors:  F Cremer; J Dommes; C Van de Walle; G Bernier
Journal:  Plant Physiol       Date:  1990-12       Impact factor: 8.340

2.  Avoiding acidic region streaking in two-dimensional gel electrophoresis: case study with two bacterial whole cell protein extracts.

Authors:  Arnab Roy; Umesh Varshney; Debnath Pal
Journal:  J Biosci       Date:  2014-09       Impact factor: 1.826

3.  Gibberellin-Induced Changes in the Populations of Translatable mRNAs and Accumulated Polypeptides in Dwarfs of Maize and Pea.

Authors:  J Chory; D F Voytas; N E Olszewski; F M Ausubel
Journal:  Plant Physiol       Date:  1987-01       Impact factor: 8.340

4.  New polypeptides and in-vitro-translatable mRNAs are produced by phosphate-starved cells of the unicellular algaChlamydomonas reinhardtii.

Authors:  F Dumont; R Loppes; P Kremers
Journal:  Planta       Date:  1990-11       Impact factor: 4.116

5.  Assay of plant proteins with bicinchoninic acid for high resolution two-dimensional polyacrylamide gel electrophoresis.

Authors:  A R Orr; B A Wagner; C T Howard; O A Schwartz
Journal:  Plant Cell Rep       Date:  1988-12       Impact factor: 4.570

6.  Evaluation of sample preparation methods for mass spectrometry-based proteomic analysis of barley leaves.

Authors:  Wei-Qing Wang; Ole Nørregaard Jensen; Ian Max Møller; Kim H Hebelstrup; Adelina Rogowska-Wrzesinska
Journal:  Plant Methods       Date:  2018-08-25       Impact factor: 4.993

Review 7.  Bringing New Methods to the Seed Proteomics Platform: Challenges and Perspectives.

Authors:  Galina Smolikova; Daria Gorbach; Elena Lukasheva; Gregory Mavropolo-Stolyarenko; Tatiana Bilova; Alena Soboleva; Alexander Tsarev; Ekaterina Romanovskaya; Ekaterina Podolskaya; Vladimir Zhukov; Igor Tikhonovich; Sergei Medvedev; Wolfgang Hoehenwarter; Andrej Frolov
Journal:  Int J Mol Sci       Date:  2020-12-01       Impact factor: 5.923
  7 in total

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