| Literature DB >> 4025804 |
C S Hintz, W R Turk, N Cambon, H B Burch, P M Nemeth, O H Lowry.
Abstract
A method for branched-chain amino acid aminotransferase is described which is based on running the reaction in the reverse of the usual direction with glutamate and alpha-ketoisocaproate as substrates. The alpha-ketoglutarate generated is reduced with glutamate dehydrogenase and NADH. For sensitivity in the nanomole range, the NAD+ generated is measured directly by converting to the highly fluorescent strong alkali product. For smaller samples, down to the 0.2- to 2-pmol range, the NAD+ is amplified by enzymatic cycling.Entities:
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Year: 1985 PMID： 4025804 DOI： 10.1016/0003-2697(85)90561-5
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365