Literature DB >> 4024729

Natural heterogeneity of shape, infectivity and protein composition in an influenza A (H3N2) virus preparation.

R W Ruigrok, P C Krijgsman, F M de Ronde-Verloop, J C de Jong.   

Abstract

Influenza A (X31) virus was purified over a zonal sucrose gradient. The resulting gradient fractions were examined by electron microscopy, HA and infectivity titrations and gel electrophoresis. The fractions containing a homogeneous suspension of spherical particles had the highest infectivity per amount of viral protein and a much higher HA:M ratio than the unfractionated preparation. These results explain differences in the proportions of HA and M protein we have reported elsewhere on monodisperse virus suspensions compared with earlier measurements on crude suspensions.

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Year:  1985        PMID： 4024729     DOI： 10.1016/0168-1702(85)90042-5

Source DB:  PubMed          Journal:  Virus Res        ISSN： 0168-1702            Impact factor:   3.303


  11 in total

1.  Influenza virus matrix protein is the major driving force in virus budding.

Authors:  P Gómez-Puertas; C Albo; E Pérez-Pastrana; A Vivo; A Portela
Journal:  J Virol       Date:  2000-12       Impact factor: 5.103

2.  Thermal denaturation of influenza virus and its relationship to membrane fusion.

Authors:  Richard M Epand; Raquel F Epand
Journal:  Biochem J       Date:  2002-08-01       Impact factor: 3.857

3.  Restriction of viral replication by mutation of the influenza virus matrix protein.

Authors:  Teresa Liu; Zhiping Ye
Journal:  J Virol       Date:  2002-12       Impact factor: 5.103

4.  The genetic aspects of influenza virus filamentous particle formation.

Authors:  M A Kuznetsova; N V Kaverin
Journal:  Arch Virol       Date:  1991       Impact factor: 2.574

5.  Quantitative electron microscopy and fluorescence spectroscopy of the membrane distribution of influenza hemagglutinin.

Authors:  Samuel T Hess; Mukesh Kumar; Anil Verma; Jane Farrington; Anne Kenworthy; Joshua Zimmerberg
Journal:  J Cell Biol       Date:  2005-06-20       Impact factor: 10.539

6.  Dynamic clustered distribution of hemagglutinin resolved at 40 nm in living cell membranes discriminates between raft theories.

Authors:  Samuel T Hess; Travis J Gould; Manasa V Gudheti; Sarah A Maas; Kevin D Mills; Joshua Zimmerberg
Journal:  Proc Natl Acad Sci U S A       Date:  2007-10-24       Impact factor: 11.205

7.  Measuring pKa of activation and pKi of inactivation for influenza hemagglutinin from kinetics of membrane fusion of virions and of HA expressing cells.

Authors:  Aditya Mittal; Tong Shangguan; Joe Bentz
Journal:  Biophys J       Date:  2002-11       Impact factor: 4.033

8.  A negative stain for electron microscopic tomography.

Authors:  Andrea Fera; Jane E Farrington; Joshua Zimmerberg; Thomas S Reese
Journal:  Microsc Microanal       Date:  2012-02-27       Impact factor: 4.127

9.  Morphology of influenza B/Lee/40 determined by cryo-electron microscopy.

Authors:  Garrett Katz; Younes Benkarroum; Hui Wei; William J Rice; Doris Bucher; Alexandra Alimova; Al Katz; Joanna Klukowska; Gabor T Herman; Paul Gottlieb
Journal:  PLoS One       Date:  2014-02-06       Impact factor: 3.240

10.  Studies of an influenza A virus temperature-sensitive mutant identify a late role for NP in the formation of infectious virions.

Authors:  Sarah L Noton; Martha Simpson-Holley; Elizabeth Medcalf; Helen M Wise; Edward C Hutchinson; John W McCauley; Paul Digard
Journal:  J Virol       Date:  2008-11-05       Impact factor: 5.103
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