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Literature DB >> 402108

Purification and specificity of bacteriolytic enzyme I from Hartmannella glebae.

J M Upadhyay, B A Mares, D M Hemelt, P G Rivet.

Abstract

A soil amoeba (Hartmannella glebae), when grown in conjunction with Enterobacter aerogenes and Alcaligenes faecalis, produced two enzymes. Enzyme I was purified by gel filtration on Sephadex G-100 and chromatography on diethylaminoethyl-cellulose. It is a basic protein. The analysis of the enzymic digest of the cell walls of Micrococcus lysodeikticus after reduction and acid hydrolysis showed that the enzyme cleaved the glycosidic bond between acetylmuramic acid and acetylglucosamine of the peptidoglycan moiety of the cell walls. The enzyme is identified as endo-beta-N-acetylmuramidase.

Entities: Chemical Species

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Year: 1977 PMID： 402108 PMCID： PMC170564 DOI： 10.1128/aem.33.1.1-5.1977

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

12 in total

1. Acetylhexosamine compounds enzymically released from Micrococcus lysodeikticus cell walls. III. The structure of DI- and tetra-saccharides released from cell walls by lysozyme and Streptomyces F1 enzyme.

Authors: M R SALTON; J M GHUYSEN
Journal: Biochim Biophys Acta Date: 1960-12-04

2. Bacteriolytic activities of the free-living soil amoebae, Acanthamoeba castellanii, Acanthamoeba polyphaga and Hartmannella vermiformis.

Authors: P H Weekers; A M Engelberts; G D Vogels
Journal: Antonie Van Leeuwenhoek Date: 1995-10 Impact factor: 2.271

2 in total

Purification and specificity of bacteriolytic enzyme I from Hartmannella glebae.

1. Acetylhexosamine compounds enzymically released from Micrococcus lysodeikticus cell walls. III. The structure of DI- and tetra-saccharides released from cell walls by lysozyme and Streptomyces F1 enzyme.

2. An improved method for the detection of N-acetylamino sugars on paper chromatograms.

3. A chromatographic investigation of pancreatic ribonuclease.

4. Protein measurement with the Folin phenol reagent.

5. [Enrichment and characterization of a bacteriolytic amoebic enzyme].

6. Action of an N-acetylmuramidase from a Limax amoeba on the murein of Proteus mirabilis.

7. Purification and properties of a bacteriolytic enzyme from a soil amoeba Hartmannella glebae.

8. Bacteriolytic enzyme produced by Acanthamoeba castellanii.

9. Growth and bacteriolytic activity of a soil amoeba, Hartmannella glebae.

10. Effect of lytic enzymes of Acanthamoeba castellanii on bacterial cell walls.

1. Specificity of bacteriolytic enzyme II from a soil amoeba, Hartmannella glebae.

2. Bacteriolytic activities of the free-living soil amoebae, Acanthamoeba castellanii, Acanthamoeba polyphaga and Hartmannella vermiformis.