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Literature DB >> 4020859

Unbiased estimation of particle density in the tandem scanning reflected light microscope.

Abstract

The tandem scanning reflected light microscope has the property of being able to obtain information from 'inside' solid objects by taking a thin optical section at the focal plane of the objective lens. This plane can be focused up and down through the specimen. We describe an unbiased 3-D counting rule for the TSRLM, which is applied to the estimation of osteocyte lacunar density in whole bone. This is shown to be an extremely efficient way of making such an estimate. Further possibilities for the application of the microscope in the field of stereology are discussed.

Mesh：

Year: 1985 PMID： 4020859 DOI： 10.1111/j.1365-2818.1985.tb02613.x

Source DB: PubMed Journal: J Microsc ISSN： 0022-2720 Impact factor: 1.758

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Cited

15 in total

1. Extending unbiased stereology of brain ultrastructure to three-dimensional volumes.

Authors: J C Fiala; K M Harris
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2. Proceedings of the Anatomical Society of Great Britain and Ireland. Papers presented at the symposium on Multidimensional Morphology Analysis held during the summer meeting of the Society. 11-13 July 1989, Liverpool. Abstracts.

Authors:
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Review 3. Efficient quantitative morphological phenotyping of genetically altered organisms using stereology.

Authors: John Milton Lucocq
Journal: Transgenic Res Date: 2006-11-14 Impact factor: 2.788

Review 4. If you assume, you can make an ass out of u and me': a decade of the disector for stereological counting of particles in 3D space.

Authors: T M Mayhew; H J Gundersen
Journal: J Anat Date: 1996-02 Impact factor: 2.610

5. IFN-gamma promotes complement expression and attenuates amyloid plaque deposition in amyloid beta precursor protein transgenic mice.

Authors: Paramita Chakrabarty; Carolina Ceballos-Diaz; Amanda Beccard; Christopher Janus; Dennis Dickson; Todd E Golde; Pritam Das
Journal: J Immunol Date: 2010-04-05 Impact factor: 5.422

10. Rhinencephalic glial cell nests and their possible role in glioma formation: morphometric studies do not reveal significant differences between brachycephalic and dolichocephalic dogs.

Authors: G Obermaier; A Reihlen; E Dahme; S Weis
Journal: Acta Neuropathol Date: 1995 Impact factor: 17.088

Unbiased estimation of particle density in the tandem scanning reflected light microscope.

1. Extending unbiased stereology of brain ultrastructure to three-dimensional volumes.

2. Proceedings of the Anatomical Society of Great Britain and Ireland. Papers presented at the symposium on Multidimensional Morphology Analysis held during the summer meeting of the Society. 11-13 July 1989, Liverpool. Abstracts.

Review 3. Efficient quantitative morphological phenotyping of genetically altered organisms using stereology.

Review 4. If you assume, you can make an ass out of u and me': a decade of the disector for stereological counting of particles in 3D space.

5. IFN-gamma promotes complement expression and attenuates amyloid plaque deposition in amyloid beta precursor protein transgenic mice.

6. Volume estimation of biological objects by systematic sections.

7. Determination of the numerical density of perforated synapses in rat neocortex.

8. Densities and Laminar Distributions of Kv3.1b-, PV-, GABA-, and SMI-32-Immunoreactive Neurons in Macaque Area V1.

9. Practical Modeling Concepts for Connective Tissue Stem Cell and Progenitor Compartment Kinetics.

10. Rhinencephalic glial cell nests and their possible role in glioma formation: morphometric studies do not reveal significant differences between brachycephalic and dolichocephalic dogs.