| Literature DB >> 4019521 |
M Tal, A Silberstein, E Nusser.
Abstract
Dimethyl sulfoxide was found to be effective for extraction of Coomassie Brilliant Blue R-250 (Coomassie R) from stained proteins on polyacrylamide gel slices. A good correlation was found between the ability of different proteins to bind Coomassie R and their capacity for interaction with Coomassie Brilliant Blue G-250 (Coomassie G) in solution. Scatchard analysis showed that the number of Coomassie R ligands bound to each protein molecule is approximately proportional to the number of positive charges on the protein, about 1.5-3 dye molecules/charge.Entities:
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Year: 1985 PMID： 4019521
Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157