Literature DB >> 4019415

Localization of the enantiozymes of 6-hydroxy-nicotine oxidase in Arthrobacter oxidans by electron immunochemistry.

J R Swafford, H C Reeves, R Brandsch.   

Abstract

During the course of growth of Arthrobacter oxidans, induction of the enantiozymes 6-hydroxy-D-nicotine oxidase and 6-hydroxy-L-nicotine oxidase occurred in the presence of DL-nicotine. Cryoultramicrotomed sections obtained from cells grown to stationary phase were gold immunolabeled. The results obtained demonstrate that both enzymes are localized in the cytoplasm.

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Year:  1985        PMID： 4019415      PMCID： PMC219194          DOI： 10.1128/jb.163.2.792-795.1985

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  9 in total

1.  [On the decomposition of nicotine by bacterial enzymes. II. Isolation and characterization of a nicotine-splitting soil bacterium].

Authors:  H EBERWEIN; F A GRIES; K DECKER
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1961-05-03

2.  D- and L-6-hydroxynicotine oxidase, enantiozymes of Arthrobacter oxidans.

Authors:  K Decker; V D Dai; H Möhler; M Brühmüller
Journal:  Z Naturforsch B       Date:  1972-09       Impact factor: 1.047

3.  [Mechanism of induction of nicotine degrading enzymes in Arthrobacter oxydans].

Authors:  M Gloger; K Decker
Journal:  Z Naturforsch B       Date:  1969-08       Impact factor: 1.047

4.  A low-viscosity epoxy resin embedding medium for electron microscopy.

Authors:  A R Spurr
Journal:  J Ultrastruct Res       Date:  1969-01

5.  Covalently bound flavin in D-6-hydroxynicotine oxidase from Arthrobacter oxidans. Identification of the 8 -(N-3-histidyl)-riboflavin-linkage between FAD and apoenzyme.

Authors:  H Möhler; M Brühmüller; K Decker
Journal:  Eur J Biochem       Date:  1972-08-18

6.  Mechanism and specifcity of L- and D-6-hydroxynicotine oxidase.

Authors:  K Decker; V D Dai
Journal:  Eur J Biochem       Date:  1967-12

7.  Induction and purification of stereospecific nicotine oxidizing enzymes from Arthrobacter oxidans.

Authors:  K Decker; H Bleeg
Journal:  Biochim Biophys Acta       Date:  1965-08-24

8.  Immunochemical localization of NADP-specific isocitrate dehydrogenase in Escherichia coli.

Authors:  J R Swafford; P J Malloy; H C Reeves
Journal:  Science       Date:  1983-07-15       Impact factor: 47.728

9.  The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.

Authors:  E S REYNOLDS
Journal:  J Cell Biol       Date:  1963-04       Impact factor: 10.539
  9 in total
  1 in total

1.  Immunocytochemical ultrastructural analysis of chromatophore membrane formation in Rhodospirillum rubrum.

Authors:  S M Crook; S B Treml; M L Collins
Journal:  J Bacteriol       Date:  1986-07       Impact factor: 3.490
  1 in total

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