Literature DB >> 4019248

Chemical and histochemical studies of normal and diseased gastrointestinal tract. IV. A comparison of histochemical and chemical methods for the estimation of side chain O-acylated sialic acids.

P E Reid, C W Ramey, D A Owen, W L Dunn, D A Lazosky, M G Clay.   

Abstract

Statistically significant correlations were obtained between a chemical assay for the proportion of colonic epithelial glycoprotein sialic acids with side chain O-acyl substituents and two histochemical methods, the PBT-KOH-PAS sequence (rs = 0.7485 for N = 31, P = 0.01, one-sided test) and the PAPT-KOH-Bh-PAS procedure (rs = 0.7024 for N = 34). A positive correlation (rs = 0.8654 for N = 30, P = 0.01) was also obtained between the results of the two histochemical procedures. It is concluded that, on average, histochemical observations are a reliable semiquantitative comparative method for the estimation of side chain O-acetylated sialic acids.

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Year:  1985        PMID： 4019248     DOI： 10.1007/bf01003217

Source DB:  PubMed          Journal:  Histochem J        ISSN： 0018-2214


  7 in total

1.  Differences in chemical composition between the epithelial glycoproteins of the upper and lower halves of rat colon.

Authors:  P E Reid; C F Culling; W L Dunn; C W Ramey; M G Clay
Journal:  Can J Biochem       Date:  1975-12

2.  The histochemical demonstration of O-acylated sialic acid in gastrointestinal mucins. Their association with the potassium hydroxide-periodic acid-schiff effect.

Authors:  C F Culling; P E Reid; M G Clay; W L Dunn
Journal:  J Histochem Cytochem       Date:  1974-08       Impact factor: 2.479

3.  A correlative chemical and histochemical study of the O-acetylated sialic acids of human colonic epithelial glycoproteins in formalin fixed paraffin embedded tissues.

Authors:  P E Reid; C F Culling; W L Dunn; M G Clay; C W Ramey
Journal:  J Histochem Cytochem       Date:  1978-12       Impact factor: 2.479

4.  Chemical and histochemical studies of normal and diseased human gastrointestinal tract. I. A comparison between histologically normal colon, colonic tumours, ulcerative colitis and diverticular disease of the colon.

Authors:  P E Reid; C F Culling; W L Dunn; C W Ramey; M G Clay
Journal:  Histochem J       Date:  1984-03

5.  Saponification-induced increase in the periodic acid-Schiff reaction in the gastrointestinal tract. Mechanism and distribution of the reactive substance.

Authors:  P E Reid; C F Culling; W L Dunn
Journal:  J Histochem Cytochem       Date:  1973-05       Impact factor: 2.479

6.  Differences between the O-acetylated sialic acids of the epithelial mucins of human colonic tumors and normal controls: a correlative chemical and histochemical study.

Authors:  P E Reid; C F Culling; W L Dunn; C W Ramey; A B Magil; M G Clay
Journal:  J Histochem Cytochem       Date:  1980-03       Impact factor: 2.479

7.  Histochemical identification of side chain substituted O-acylated sialic acids: the PAT-KOH-Bh-PAS and the PAPT-KOH-Bh-PAS procedures.

Authors:  P E Reid; W L Dunn; C W Ramey; E Coret; L Trueman; M G Clay
Journal:  Histochem J       Date:  1984-06
  7 in total
  4 in total

1.  Reduction of sialic acid O-acetylation in human colonic mucins in the adenoma-carcinoma sequence.

Authors:  A P Corfield; N Myerscough; B F Warren; P Durdey; C Paraskeva; R Schauer
Journal:  Glycoconj J       Date:  1999-06       Impact factor: 2.916

2.  Histochemical procedures for the simultaneous visualization of sialic acid, its side chain O-acyl variants and O-sulphate ester.

Authors:  P E Reid; D A Owen; C W Ramey; W L Dunn; M G Clay; E A Jones
Journal:  Histochem J       Date:  1985-01

3.  Chemical and histochemical studies of normal and diseased human gastrointestinal tract. V. A differential diagnostic method for the histochemical classification of glycoproteins.

Authors:  P E Reid; D A Owen; C W Ramey; W L Dunn; E A Jones; D A Lazosky; E Allen; C M Park; M G Clay
Journal:  Histochem J       Date:  1985-08

4.  Binding specificity of influenza C-virus to variably O-acetylated glycoconjugates and its use for histochemical detection of N-acetyl-9-O-acetylneuraminic acid in mammalian tissues.

Authors:  G Harms; G Reuter; A P Corfield; R Schauer
Journal:  Glycoconj J       Date:  1996-08       Impact factor: 2.916
  4 in total

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