Literature DB >> 4018305

Hybridoma cultivation in defined serum-free media: growth-supporting substances. I. Transferrin.

J Kovár, F Franĕk.   

Abstract

Effect of transferrin and effect of transferrin omission on growth of hybridomas in serum-free medium were studied. Transferrin at concentration 0.5 microgram/ml and higher concentrations stimulated growth of hybridoma cells. The optimum growth-supporting concentration of transferrin was 5 micrograms/ml. Omission of transferrin led to death of hybridoma T3-03 cells within 3-4 days. Omission of transferrin for a period of only 24 h damaged cells so severely, that they were unable to recover fully.

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Year:  1985        PMID： 4018305

Source DB:  PubMed          Journal:  Folia Biol (Praha)        ISSN： 0015-5500            Impact factor:   0.906


  3 in total

1.  Application of a statistical design to the optimization of culture medium for recombinant interferon-gamma production by Chinese hamster ovary cells.

Authors:  P M Castro; P M Hayter; A P Ison; A T Bull
Journal:  Appl Microbiol Biotechnol       Date:  1992-10       Impact factor: 4.813

2.  Bioactive recombinant human lactoferrin, derived from rice, stimulates mammalian cell growth.

Authors:  N Huang; D Bethell; C Card; J Cornish; T Marchbank; D Wyatt; K Mabery; R Playford
Journal:  In Vitro Cell Dev Biol Anim       Date:  2008-09-19       Impact factor: 2.416

3.  Growth of human tumor cell lines in transferrin-free, low-iron medium.

Authors:  V Neumannova; D R Richardson; K Kriegerbeckova; J Kovar
Journal:  In Vitro Cell Dev Biol Anim       Date:  1995-09       Impact factor: 2.416
  3 in total

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