Literature DB >> 4018235

Improved separation at low temperature of glycoproteins by Con A-Sepharose affinity chromatography in the presence of sodium dodecyl sulfate (SDS).

S Aono, H Sato, R Semba, S Kashiwamata.   

Abstract

The Con A-Sepharose affinity chromatography of glycoproteins was even more effective at 4 degrees C than that at room temperature (26-28 degrees C) in the presence of sodium dodecyl sulfate (SDS). Application of this methodology to the separation of several glycoproteins from SDS-solubilized membrane proteins in rat cerebellum, including a glycoprotein characteristic of the Purkinje cells, was successful.

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Year:  1985        PMID： 4018235     DOI： 10.1007/bf01952156

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  6 in total

1.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

2.  A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels.

Authors:  B R Oakley; D R Kirsch; N R Morris
Journal:  Anal Biochem       Date:  1980-07-01       Impact factor: 3.365

3.  Anatomical, physiological and biochemical studies on the cerebellum from mutant mice. III. Protein differences associated with the weaver, staggerer and nervous mutations.

Authors:  J Mallet; M Huchet; R Pougeois; J P Changeux
Journal:  Brain Res       Date:  1976-02-20       Impact factor: 3.252

4.  Two proteins associated with cerebellar hypoplasia in jaundiced Gunn rats.

Authors:  S Aono; H Sato; R Semba; S Kashiwamata
Journal:  Neurochem Res       Date:  1983-06       Impact factor: 3.996

5.  Activities of lectins and their immobilized derivatives in detergent solutions. Implications on the use of lectin affinity chromatography for the purification of membrane glycoproteins.

Authors:  R Lotan; G Beattie; W Hubbell; G L Nicolson
Journal:  Biochemistry       Date:  1977-05-03       Impact factor: 3.162

6.  Characteristic changes of cerebellar proteins associated with cerebellar hypoplasia in jaundiced Gunn rats and the prevention of these by phototherapy.

Authors:  S Kashiwamata; S Aono; R K Semba
Journal:  Experientia       Date:  1980-10-15
  6 in total
  1 in total

1.  The generation and characterization of a rat neural cell line overexpressing the alpha2,6(N) sialyltransferase.

Authors:  K C Breen; A Potratz; N Georgopoulou; K Sandhoff
Journal:  Glycoconj J       Date:  1998-02       Impact factor: 2.916
  1 in total

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