Literature DB >> 4017747

Mass characteristics of DNA obtained from chromosomes of a human carcinoma cell line.

S K Stickel, R W Clark.   

Abstract

Sedimentation studies of DNA from chromosomes extracted from human mitotic cells showed that high-molecular-weight DNA can be obtained if cell hypotonic treatments and prolonged metaphase blocks are avoided. Two types of large double-stranded DNA were observed. One of these (Mr = 2.5 X 10(8)) appeared as a size class with characteristics reminiscent of the chromosomal DNA subunit hypothesis. However, this DNA is the decay product of larger molecules, whose minimum molecular weight is 6 X 10(8).

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Year:  1985        PMID： 4017747     DOI： 10.1007/bf00348699

Source DB:  PubMed          Journal:  Chromosoma        ISSN： 0009-5915            Impact factor:   4.316


  16 in total

1.  Length distributions of single-stranded DNA in Chinese hamster ovary cells.

Authors:  J C Hozier; J H Taylor
Journal:  J Mol Biol       Date:  1975-04-05       Impact factor: 5.469

2.  Anomalies in sedimentation. IV. Decrease in sedimentation coefficients of chains at high fields.

Authors:  B H Zimm
Journal:  Biophys Chem       Date:  1974-04       Impact factor: 2.352

3.  Mammalian metaphase chromosomes with high molecular weight DNA isolated at pH 10.5.

Authors:  W Wray; E Stubblefield; R Humphrey
Journal:  Nat New Biol       Date:  1972-08-23

4.  Conformation of DNA in alkaline sucrose: the subunit hypothesis in mammalian cells.

Authors:  J E Cleaver
Journal:  Biochem Biophys Res Commun       Date:  1974-07-10       Impact factor: 3.575

5.  Chromosome-sized DNA molecules from Drosophila.

Authors:  R Kavenoff; B H Zimm
Journal:  Chromosoma       Date:  1973       Impact factor: 4.316

6.  A new method for the rapid isolation of chromosomes, mitotic apparatus, or nuclei from mammalian fibroblasts at near neutral pH.

Authors:  W Wray; E Stubblefield
Journal:  Exp Cell Res       Date:  1970-03       Impact factor: 3.905

7.  High-molecular-weight DNA and the sedimentation coefficient: a new perspective based on DNA from T7 bacteriophage and two novel forms of T4 bacteriophage.

Authors:  R W Clark; G H Wever; J S Wiberg
Journal:  J Virol       Date:  1980-01       Impact factor: 5.103

8.  Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions.

Authors:  L A Quinn; G E Moore; R T Morgan; L K Woods
Journal:  Cancer Res       Date:  1979-12       Impact factor: 12.701

9.  Characterization of macromolecules by constant velocity sedimentation.

Authors:  H Noll
Journal:  Nature       Date:  1967-07-22       Impact factor: 49.962

10.  On the size of the DNA in the mammalian chromosome. Structural subunits.

Authors:  J T Lett; E S Klucis; C Sun
Journal:  Biophys J       Date:  1970-03       Impact factor: 4.033
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  1 in total

1.  Natural size classes in DNA from Chinese hamster metaphase chromosomes.

Authors:  J L Murata-Collins; R W Clark
Journal:  Chromosoma       Date:  1987       Impact factor: 4.316
  1 in total

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