Literature DB >> 4014674

Proteins transferred to nitrocellulose for use as immunogens.

K A Knudsen.   

Abstract

Proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and were eluted efficiently from the gel by transfer to nitrocellulose paper. The protein-bearing nitrocellulose was solubilized with dimethyl sulfoxide and used as an immunogen in rabbits. Polyclonal antibodies to the proteins were generated.

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Year:  1985        PMID： 4014674     DOI： 10.1016/0003-2697(85)90273-8

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  29 in total

1.  Sequence of cDNA for rat cystathionine gamma-lyase and comparison of deduced amino acid sequence with related Escherichia coli enzymes.

Authors:  P F Erickson; I H Maxwell; L J Su; M Baumann; L M Glode
Journal:  Biochem J       Date:  1990-07-15       Impact factor: 3.857

2.  In indirect flight muscles Drosophila projectin has a short PEVK domain, and its NH2-terminus is embedded at the Z-band.

Authors:  Agnes Ayme-Southgate; Judith Saide; Richard Southgate; Christophe Bounaix; Anthony Cammarato; Sunita Patel; Catherine Wussler
Journal:  J Muscle Res Cell Motil       Date:  2005       Impact factor: 2.698

3.  Complex formation of human T-cell leukemia virus type I p40tax transactivator with cellular polypeptides.

Authors:  K Nagata; Y Ide; T Takagi; K Ohtani; M Aoshima; H Tozawa; M Nakamura; K Sugamura
Journal:  J Virol       Date:  1992-02       Impact factor: 5.103

4.  A novel plant peptidyl-prolyl-cis-trans-isomerase (PPIase): cDNA cloning, structural analysis, enzymatic activity and expression.

Authors:  O Blecher; N Erel; I Callebaut; K Aviezer; A Breiman
Journal:  Plant Mol Biol       Date:  1996-11       Impact factor: 4.076

5.  Protein import into mitochondria: ATP-dependent protein translocation activity in a submitochondrial fraction enriched in membrane contact sites and specific proteins.

Authors:  L Pon; T Moll; D Vestweber; B Marshallsay; G Schatz
Journal:  J Cell Biol       Date:  1989-12       Impact factor: 10.539

6.  Characterization of an antibody directed against a 128 kDa glycoprotein involved in the thrombogenicity of the elastin-associated microfibrils.

Authors:  F Fauvel-Lafeve; P Picard; G Godeau; Y J Legrand
Journal:  Biochem J       Date:  1988-10-01       Impact factor: 3.857

7.  Characterization and gene structure of a novel retinoblastoma-protein-associated protein similar to the transcription regulator TFII-I.

Authors:  X Yan; X Zhao; M Qian; N Guo; X Gong; X Zhu
Journal:  Biochem J       Date:  2000-02-01       Impact factor: 3.857

8.  Characterization of specific immune responses of mice inoculated with recombinant vaccinia virus expressing an 18-kilodalton outer membrane protein of Brucella abortus.

Authors:  R Vemulapalli; S Cravero; C L Calvert; T E Toth; N Sriranganathan; S M Boyle; O L Rossetti; G G Schurig
Journal:  Clin Diagn Lab Immunol       Date:  2000-01

9.  Isolation and sequence determination of a cDNA clone related to human cytochrome P-450 nifedipine oxidase.

Authors:  P H Beaune; D R Umbenhauer; R W Bork; R S Lloyd; F P Guengerich
Journal:  Proc Natl Acad Sci U S A       Date:  1986-11       Impact factor: 11.205

10.  "Bundle blot" purification and initial protein characterization of hair cell stereocilia.

Authors:  G M Shepherd; B A Barres; D P Corey
Journal:  Proc Natl Acad Sci U S A       Date:  1989-07       Impact factor: 11.205
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