Literature DB >> 4014670

Synthesis, storage, and stability of [4-14C]oxaloacetic acid.

M D Hatch, H W Heldt.   

Abstract

A simple procedure for preparing [4-14C]oxaloacetic acid based on the reaction between [14C]HCO-3 and phosphoenolpyruvate catalyzed by phosphoenolpyruvate carboxylase is described. A simple method for preparing highly purified phosphoenolpyruvate carboxylase from maize leaves is described and the degradation of oxaloacetate under conditions of varying pH and divalent metal ion concentration is reported on. [4-14C]Oxaloacetic acid is stable for several months in 0.1 M HCl solution at -80 degrees C.

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Year:  1985        PMID： 4014670     DOI： 10.1016/0003-2697(85)90379-3

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  10 in total

1.  Isolation and partial characterization of the glutamate/aspartate transporter from pea leaf mitochondria using a specific monoclonal antibody.

Authors:  J Vivekananda; D J Oliver
Journal:  Plant Physiol       Date:  1989-09       Impact factor: 8.340

2.  Purification, oligomerization state and malate sensitivity of maize leaf phosphoenolpyruvate carboxylase.

Authors:  G A McNaughton; C A Fewson; M B Wilkins; H G Nimmo
Journal:  Biochem J       Date:  1989-07-15       Impact factor: 3.857

3.  Mechanism of c(4) photosynthesis: the size and composition of the inorganic carbon pool in bundle sheath cells.

Authors:  R T Furbank; M D Hatch
Journal:  Plant Physiol       Date:  1987-12       Impact factor: 8.340

4.  Genetic, Genomic, and Transcriptomic Studies of Pyruvate Metabolism in Methanosarcina barkeri Fusaro.

Authors:  Madeline M López Muñoz; Peter Schönheit; William W Metcalf
Journal:  J Bacteriol       Date:  2015-09-08       Impact factor: 3.490

5.  Salt Stress Increases the Level of Translatable mRNA for Phosphoenolpyruvate Carboxylase in Mesembryanthemum crystallinum.

Authors:  J A Ostrem; S W Olson; J M Schmitt; H J Bohnert
Journal:  Plant Physiol       Date:  1987-08       Impact factor: 8.340

6.  The simultaneous determination of carbon dioxide release and oxygen uptake in suspensions of plant leaf mitochondria oxidizing glycine.

Authors:  R M Lilley; H Ebbighausen; H W Heldt
Journal:  Plant Physiol       Date:  1987-02       Impact factor: 8.340

7.  Isolation and characterization of a gene coding for a novel aspartate aminotransferase from Rhizobium meliloti.

Authors:  J R Alfano; M L Kahn
Journal:  J Bacteriol       Date:  1993-07       Impact factor: 3.490

8.  Multiple active site histidine protonation states in Acetobacter aceti N5-carboxyaminoimidazole ribonucleotide mutase detected by REDOR NMR.

Authors:  Jacob Schaefer; Hong Jiang; Aaron E Ransome; T Joseph Kappock
Journal:  Biochemistry       Date:  2007-07-27       Impact factor: 3.162

9.  Role of aminotransferases in glutamate metabolism of human erythrocytes.

Authors:  James J Ellinger; Ian A Lewis; John L Markley
Journal:  J Biomol NMR       Date:  2011-03-06       Impact factor: 2.835

10.  Studies on the Interaction of the Histone Demethylase KDM5B with Tricarboxylic Acid Cycle Intermediates.

Authors:  Hanna Tarhonskaya; Radosław P Nowak; Catrine Johansson; Aleksandra Szykowska; Anthony Tumber; Rebecca L Hancock; Pauline Lang; Emily Flashman; Udo Oppermann; Christopher J Schofield; Akane Kawamura
Journal:  J Mol Biol       Date:  2017-08-18       Impact factor: 5.469
  10 in total

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