Literature DB >> 4007493

Cloning of the nodulation (nod) genes of Rhizobium phaseoli and their homology to R. leguminosarum nod DNA.

J W Lamb, J A Downie, A W Johnston.   

Abstract

In Rhizobium phaseoli strain 8002, a large indigenous plasmid, pRP2JI, had previously been shown to carry many of the genes necessary for the induction of nitrogen-fixing nodules on Phaseolus beans. A cosmid clone library was constructed using DNA from strain 8002. From this library, two overlapping recombinant plasmids (pIJ1097 and pIJ1098) were isolated which spanned about 43 kb of pRP2JI DNA. These plasmids could restore nodulation to some, but not all nodulation-deficient strains of R. phaseoli, indicating that the nodulation genes were not clustered within one small region of pRP2JI. The cloned R. phaseoli nodulation region shared extensive DNA homology with the nodulation genes of R. leguminosarum, and on the basis of DNA hybridization, the nitrogenase genes were found to be within 10 kb of the R. phaseoli nodulation genes. Close to the nodulation genes of R. phaseoli was located a sequence that was repeated on pRP2JI but which was not present elsewhere in the genome of strain 8002.

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Year:  1985        PMID： 4007493     DOI： 10.1016/0378-1119(85)90132-5

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  8 in total

1.  Assignment of biological functions to specific plasmids in a local isolate of Rhizobium cicer.

Authors:  E E Yildiz; G Ozcengiz; B Içgen; N G Alaeddinoğlu
Journal:  Folia Microbiol (Praha)       Date:  1999       Impact factor: 2.099

2.  Evidence that DNA involved in the expression of nodulation (nod) genes in Rhizobium binds to the product of the regulatory gene nodD.

Authors:  G F Hong; J E Burn; A W Johnston
Journal:  Nucleic Acids Res       Date:  1987-12-10       Impact factor: 16.971

3.  N-acyl-homoserine lactone inhibition of rhizobial growth is mediated by two quorum-sensing genes that regulate plasmid transfer.

Authors:  A Wilkinson; V Danino; F Wisniewski-Dyé; J K Lithgow; J A Downie
Journal:  J Bacteriol       Date:  2002-08       Impact factor: 3.490

4.  The cin and rai quorum-sensing regulatory systems in Rhizobium leguminosarum are coordinated by ExpR and CinS, a small regulatory protein coexpressed with CinI.

Authors:  Anne Edwards; Marijke Frederix; Florence Wisniewski-Dyé; Jacob Jones; Angeles Zorreguieta; J Allan Downie
Journal:  J Bacteriol       Date:  2009-03-06       Impact factor: 3.490

5.  Identification of two classes of Rhizobium phaseoli genes required for melanin synthesis, one of which is required for nitrogen fixation and activates the transcription of the other.

Authors:  D Borthakur; J W Lamb; A W Johnston
Journal:  Mol Gen Genet       Date:  1987-04

6.  Sequence of psi, a gene on the symbiotic plasmid of Rhizobium phaseoli which inhibits exopolysaccharide synthesis and nodulation and demonstration that its transcription is inhibited by psr, another gene on the symbiotic plasmid.

Authors:  D Borthakur; A W Johnston
Journal:  Mol Gen Genet       Date:  1987-04

7.  Melanin production by Rhizobium meliloti GR4 is linked to nonsymbiotic plasmid pRmeGR4b: cloning, sequencing, and expression of the tyrosinase gene mepA.

Authors:  J Mercado-Blanco; F García; M Fernández-López; J Olivares
Journal:  J Bacteriol       Date:  1993-09       Impact factor: 3.490

8.  Analysis of pss genes of Rhizobium leguminosarum required for exopolysaccharide synthesis and nodulation of peas: their primary structure and their interaction with psi and other nodulation genes.

Authors:  D Borthakur; R F Barker; J W Latchford; L Rossen; A W Johnston
Journal:  Mol Gen Genet       Date:  1988-07
  8 in total

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