Androgen and estrogen levels in the plasma of Pleurodeles waltl, Michah., during the annual cycle. I. Male cycle.

Sex steroid levels in the plasma of the male Pleurodeles waltl were determined by radioimmunoassay of blood samples taken monthly from each newt during an 18-month period. Androgen levels varied according to season with annual peaks in October-November and in March. The position of these two peaks was the same in the 2 years studied. Testosterone was the principal androgen measured, while the levels of dihydrotestosterone and 4-androstenedione were lower but fluctuated in parallel with that of testosterone. The 17 beta-estradiol levels were very low or undetectable. Androgen levels during the year were synchronous between the several newts studied. The cyclical variations in the steroid levels agreed with the morphological modifications of sexual characteristics during the annual cycle.