Literature DB >> 4005261

Eukaryotic protein synthesis initiation factor eIF-3: determination of concentration and association with ribosomes in rabbit reticulocyte and HeLa cell lysates.

G Mengod, H Trachsel.   

Abstract

Monoclonal and polyclonal antibodies against eukaryotic protein synthesis initiation factor eIF-3 were produced and used to determine the factor concentration and its association with ribosomes in rabbit reticulocyte and HeLa cell lysates. In rabbit reticulocyte lysate we found 3-5 micrograms eIF-3 per mg total protein and in HeLa cell lysate 8-15 micrograms eIF-3 per mg total protein. The initiation factor eIF-3 was found both associated with 40 S ribosomal subunits and free in the post-ribosomal supernatant. However, no eIF-3 could be detected on mono- or polyribosomes.

Entities:  

Mesh：

Substances：

Year:  1985        PMID： 4005261     DOI： 10.1016/0167-4781(85)90101-0

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  10 in total

1.  Computational modeling of eukaryotic mRNA turnover.

Authors:  D Cao; R Parker
Journal:  RNA       Date:  2001-09       Impact factor: 4.942

2.  Inhibition of polypeptide chain initiation in Daudi cells by interferons. Evidence that activity of initiation factor eIF-2 and availability of mRNA are unimpaired.

Authors:  M J Clemens; V J Tilleray
Journal:  Biochem J       Date:  1986-08-01       Impact factor: 3.857

3.  A new translational regulator with homology to eukaryotic translation initiation factor 4G.

Authors:  H Imataka; H S Olsen; N Sonenberg
Journal:  EMBO J       Date:  1997-02-17       Impact factor: 11.598

4.  p97/DAP5 is a ribosome-associated factor that facilitates protein synthesis and cell proliferation by modulating the synthesis of cell cycle proteins.

Authors:  Sang Hyun Lee; Frank McCormick
Journal:  EMBO J       Date:  2006-08-24       Impact factor: 11.598

Review 5.  Initiation of protein synthesis in mammalian cells.

Authors:  V M Pain
Journal:  Biochem J       Date:  1986-05-01       Impact factor: 3.857

6.  IRES interaction with translation initiation factors: functional characterization of novel RNA contacts with eIF3, eIF4B, and eIF4GII.

Authors:  S López de Quinto; E Lafuente; E Martínez-Salas
Journal:  RNA       Date:  2001-09       Impact factor: 4.942

7.  The mTOR/PI3K and MAPK pathways converge on eIF4B to control its phosphorylation and activity.

Authors:  David Shahbazian; Philippe P Roux; Virginie Mieulet; Michael S Cohen; Brian Raught; Jack Taunton; John W B Hershey; John Blenis; Mario Pende; Nahum Sonenberg
Journal:  EMBO J       Date:  2006-06-08       Impact factor: 11.598

8.  A region rich in aspartic acid, arginine, tyrosine, and glycine (DRYG) mediates eukaryotic initiation factor 4B (eIF4B) self-association and interaction with eIF3.

Authors:  N Méthot; M S Song; N Sonenberg
Journal:  Mol Cell Biol       Date:  1996-10       Impact factor: 4.272

9.  Reconstitution reveals the functional core of mammalian eIF3.

Authors:  Mamiko Masutani; Nahum Sonenberg; Shigeyuki Yokoyama; Hiroaki Imataka
Journal:  EMBO J       Date:  2007-06-21       Impact factor: 11.598

10.  Stoichiometric association of cap-binding protein I with translated polysomal globin mRNP.

Authors:  M Görlach; K Hilse
Journal:  EMBO J       Date:  1986-10       Impact factor: 11.598
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.