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Literature DB >> 4004249

Simple screening method for identification of nonpleiotropic mutants for exoenzyme production.

Abstract

A differential medium that distinguishes between pleiotropic and nonpleiotropic mutants for exoenzyme production has been developed for Staphylococcus simulans biovar staphylolyticus. The medium will facilitate genetic analysis of exoenzyme production by this organism. Generally useful strategies for increasing the sensitivity of indicator plates for detection of exoenzyme activities are presented.

Entities: Species

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Substances：
Culture Media

Year: 1985 PMID： 4004249 PMCID： PMC238553 DOI： 10.1128/aem.49.5.1335-1337.1985

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

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References

15 in total

1. Typing of coagulase-positive staphylococci by proteolytic activity on buffered caseinate-agar, with special reference to bacteriophage nontypable strains.

Authors: F G Martley; A W Jarvis; D F Bacon; R C Lawrence
Journal: Infect Immun Date: 1970-10 Impact factor: 3.441

2. Pleiotropic mutations in Serratia marcescens which increase the synthesis of certain exocellular proteins and the rate of spontaneous prophage induction.

Authors: U Winkler; K Timmis
Journal: Mol Gen Genet Date: 1973-08-17

3. Bacteriolytic enzymes from staphylococci.

Authors:
Journal: Contrib Microbiol Immunol Date: 1973

4. Phospholipase C regulatory mutation of Pseudomonas aeruginosa that results in constitutive synthesis of several phosphate-repressible proteins.

Authors: G L Gray; R M Berka; M L Vasil
Journal: J Bacteriol Date: 1982-06 Impact factor: 3.490

Simple screening method for identification of nonpleiotropic mutants for exoenzyme production.

1. Typing of coagulase-positive staphylococci by proteolytic activity on buffered caseinate-agar, with special reference to bacteriophage nontypable strains.

2. Pleiotropic mutations in Serratia marcescens which increase the synthesis of certain exocellular proteins and the rate of spontaneous prophage induction.

3. Bacteriolytic enzymes from staphylococci.

4. Phospholipase C regulatory mutation of Pseudomonas aeruginosa that results in constitutive synthesis of several phosphate-repressible proteins.

5. The characteristics of extracellular protein secretion by Staphylococcus staphylolyticus.

6. Chitinase-overproducing mutant of Serratia marcescens.

7. Isolation of mutants of Staphylococcus aureus lacking extracellular nuclease activity.

8. Membrane mutations and production of enterotoxin B and alpha hemolysin in Staphylococcus aureus.

9. Pleiotropic alteration of activities of several toxins and enzymes in mutants of Staphylococcus aureus.

10. Coordinate production of three exoenzymes of Staphylococcus staphylolyticus.