Literature DB >> 4001942

The mechanisms of irreversible enzyme inactivation at 100C.

T J Ahern, A M Klibanov.   

Abstract

The mechanism of irreversible thermoinactivation of an enzyme has been quantitatively elucidated in the pH range relevant to enzymatic catalysis. The processes causing irreversible inactivation of hen egg-white lysozyme at 100 degrees C are deamidation of asparagine residues, hydrolysis of peptide bonds at aspartic acid residues., destruction of disulfide bonds, and formation of incorrect (scrambled) structures; their relative contributions depend of the pH.

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Year:  1985        PMID： 4001942     DOI： 10.1126/science.4001942

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  57 in total

Review 1.  Hyperthermophilic enzymes: sources, uses, and molecular mechanisms for thermostability.

Authors:  C Vieille; G J Zeikus
Journal:  Microbiol Mol Biol Rev       Date:  2001-03       Impact factor: 11.056

2.  Chemical pathways of peptide degradation. III. Effect of primary sequence on the pathways of deamidation of asparaginyl residues in hexapeptides.

Authors:  K Patel; R T Borchardt
Journal:  Pharm Res       Date:  1990-08       Impact factor: 4.200

3.  Crystal structure of conserved hypothetical protein Aq1575 from Aquifex aeolicus.

Authors:  Dong Hae Shin; Hisao Yokota; Rosalind Kim; Sung-Hou Kim
Journal:  Proc Natl Acad Sci U S A       Date:  2002-06-11       Impact factor: 11.205

4.  Mechanisms of thermoinactivation of endoglucanase I from Trichoderma reesei QM 9414.

Authors:  J M Dominguez; C Acebal; J Jimenez; I de la Mata; R Macarron; M P Castillon
Journal:  Biochem J       Date:  1992-10-15       Impact factor: 3.857

5.  Raman spectroscopic studies of hen egg-white lysozyme at high temperatures and pressures.

Authors:  R L Remmele; P McMillan; A Bieber
Journal:  J Protein Chem       Date:  1990-08

6.  The role of intra-domain disulfide bonds in heat-induced irreversible denaturation of camelid single domain VHH antibodies.

Authors:  Yoko Akazawa-Ogawa; Koichi Uegaki; Yoshihisa Hagihara
Journal:  J Biochem       Date:  2015-08-19       Impact factor: 3.387

Review 7.  Stability of protein pharmaceuticals.

Authors:  M C Manning; K Patel; R T Borchardt
Journal:  Pharm Res       Date:  1989-11       Impact factor: 4.200

8.  Understanding the pathway and kinetics of aspartic acid isomerization in peptide mapping methods for monoclonal antibodies.

Authors:  June Kuang; Yuanqi Tao; Yuanli Song; Letha Chemmalil; Nesredin Mussa; Julia Ding; Zheng Jian Li
Journal:  Anal Bioanal Chem       Date:  2021-02-05       Impact factor: 4.142

9.  Use of monoclonal antibodies to demonstrate different sites with different functional characteristics in a bacterial lipase from Pseudomonas aeruginosa YS-7.

Authors:  N Daya-Mishne; Y Shabtai
Journal:  Appl Environ Microbiol       Date:  1992-02       Impact factor: 4.792

10.  Purification and Characterization of Two Functional Forms of Intracellular Protease PfpI from the Hyperthermophilic Archaeon Pyrococcus furiosus.

Authors:  S B Halio; M W Bauer; S Mukund; M Adams; R M Kelly
Journal:  Appl Environ Microbiol       Date:  1997-01       Impact factor: 4.792
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