Literature DB >> 3998496

FACS purification of bone marrow-derived epidermal populations in mice: Langerhans cells and Thy-1+ dendritic cells.

S Sullivan, P R Bergstresser, R E Tigelaar, J W Streilein.   

Abstract

A method was developed which allows for the separation and purification of Langerhans cells (LC) and Thy-1+ cells (Thy-1+dEC) from mouse epidermis. Epidermal cell (EC) suspensions were subjected to Ficoll separation, and the resulting interface EC were harvested. These EC were then "tagged" with the appropriate monoclonal antibody and sorted into positive and negative populations using the Fluorescence Activated Cell Sorter (FACS). Preparations of viable LC and Thy-1+dEC were obtained with 94-98% and 94-99% purities, respectively.

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Year:  1985        PMID： 3998496     DOI： 10.1111/1523-1747.ep12273454

Source DB:  PubMed          Journal:  J Invest Dermatol        ISSN： 0022-202X            Impact factor:   8.551


  16 in total

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2.  Most gamma delta T cells develop normally in beta 2-microglobulin-deficient mice.

Authors:  I Correa; M Bix; N S Liao; M Zijlstra; R Jaenisch; D Raulet
Journal:  Proc Natl Acad Sci U S A       Date:  1992-01-15       Impact factor: 11.205

Review 3.  Advances in the immunobiology of the skin. Implications for cutaneous malignancies.

Authors:  C A Romerdahl; M L Kripke
Journal:  Cancer Metastasis Rev       Date:  1986       Impact factor: 9.264

4.  Gamma delta antigen receptors of Thy-1+ dendritic epidermal cells: implications for thymic differentiation.

Authors:  J P Allison; W L Havran; D Asarnow; R E Tigelaar; P W Tucker; M Bonyhadi
Journal:  Immunol Res       Date:  1988       Impact factor: 2.829

5.  Keratinocyte expression of the type 2 interleukin 1 receptor mediates local and specific inhibition of interleukin 1-mediated inflammation.

Authors:  T Rauschmayr; R W Groves; T S Kupper
Journal:  Proc Natl Acad Sci U S A       Date:  1997-05-27       Impact factor: 11.205

6.  CCR10 is important for the development of skin-specific gammadeltaT cells by regulating their migration and location.

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Journal:  J Immunol       Date:  2010-10-11       Impact factor: 5.422

7.  Transgenic expression of survivin in keratinocytes counteracts UVB-induced apoptosis and cooperates with loss of p53.

Authors:  D Grossman; P J Kim; O P Blanc-Brude; D E Brash; S Tognin; P C Marchisio; D C Altieri
Journal:  J Clin Invest       Date:  2001-10       Impact factor: 14.808

8.  Limited diversity of T-cell receptor gamma-chain expression of murine Thy-1+ dendritic epidermal cells revealed by V gamma 3-specific monoclonal antibody.

Authors:  W L Havran; S Grell; G Duwe; J Kimura; A Wilson; A M Kruisbeek; R L O'Brien; W Born; R E Tigelaar; J P Allison
Journal:  Proc Natl Acad Sci U S A       Date:  1989-06       Impact factor: 11.205

9.  Costimulation of dendritic epidermal gammadelta T cells by a new NKG2D ligand expressed specifically in the skin.

Authors:  Michael I Whang; Nadia Guerra; David H Raulet
Journal:  J Immunol       Date:  2009-04-15       Impact factor: 5.422

10.  Recombination signal sequence-associated restriction on TCRdelta gene rearrangement affects the development of tissue-specific gammadelta T cells.

Authors:  Uzodinma N Uche; Christopher R Huber; David H Raulet; Na Xiong
Journal:  J Immunol       Date:  2009-10-15       Impact factor: 5.422
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