Literature DB >> 3994682

A sensitive spectrophotometric assay for peroxisomal acyl-CoA oxidase.

G M Small, K Burdett, M J Connock.   

Abstract

A simple spectrophotometric assay was developed for peroxisomal fatty acyl-CoA oxidase activity. The assay, based on the H2O2-dependent oxidation of leuco-dichlorofluorescein catalysed by exogenous peroxidase, is more sensitive than methods previously described. By using mouse liver samples, cofactor requirements were assessed and a linear relationship was demonstrated between dye oxidation and enzyme concentration. By using this assay on subcellular fractions, palmitoyl-CoA oxidase activity was localized for the first time in microperoxisomes of rat intestine. The assay was also adapted to measure D-amino acid oxidase activity, demonstrating the versatility of this method for measuring activity of other H2O2-producing oxidases.

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Year:  1985        PMID： 3994682      PMCID： PMC1144827          DOI： 10.1042/bj2270205

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  15 in total

1.  Analytical subcellular fractionation studies on rat liver and on isolated jejunal enterocytes with special reference to the separation of lysosomes, peroxisomes and mitochondria.

Authors:  T J Peters; H Shio
Journal:  Clin Sci Mol Med       Date:  1976-05

2.  Beta-oxidation in peroxisomes of brown adipose tissue.

Authors:  R Kramar; M Hüttinger; B Gmeiner; H Goldenberg
Journal:  Biochim Biophys Acta       Date:  1978-12-22

3.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

4.  A sensitive photometric assay for monoamine oxidase.

Authors:  H Köchli; J P Von Wartburg
Journal:  Anal Biochem       Date:  1978-01       Impact factor: 3.365

5.  Chain length specificities of peroxisomal and mitochondrial beta-oxidation in rat liver.

Authors:  D J Hryb; J F Hogg
Journal:  Biochem Biophys Res Commun       Date:  1979-04-27       Impact factor: 3.575

6.  Fatty acid oxidation by human liver peroxisomes.

Authors:  M Bronfman; N C Inestrosa; F Leighton
Journal:  Biochem Biophys Res Commun       Date:  1979-06-13       Impact factor: 3.575

7.  Enzyme activities of isolated hepatic peroxisomes from genetically lean and obese male mice.

Authors:  P A Murphy; J B Krahling; R Gee; J R Kirk; N E Tolbert
Journal:  Arch Biochem Biophys       Date:  1979-03       Impact factor: 4.013

8.  Detection of peroxisomal fatty acyl-coenzyme A oxidase activity.

Authors:  N C Inestrosa; M Bronfman; F Leighton
Journal:  Biochem J       Date:  1979-09-15       Impact factor: 3.857

9.  Palmityl-CoA oxidase: detection in several guinea pig tissues and peroxisomal localisation in mucosa, of small intestine.

Authors:  G M Small; D Brolly; M J Connock
Journal:  Life Sci       Date:  1980-11-10       Impact factor: 5.037

10.  Rat liver peroxisomes catalyze the beta oxidation of fatty acids.

Authors:  P B Lazarow
Journal:  J Biol Chem       Date:  1978-03-10       Impact factor: 5.157
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  55 in total

1.  Peroxisome distribution along the crypt-villus axis of the guinea pig small intestine.

Authors:  A N Phipps; M J Connock; P Johnson; K Burdett
Journal:  Mol Cell Biochem       Date:  2000-01       Impact factor: 3.396

2.  The activity and subcellular distribution of the peroxisomal enzyme acyl-CoA oxidase in human blood platelets.

Authors:  M Farstad; A M Bakken; R K Berge
Journal:  Biochem J       Date:  1992-09-15       Impact factor: 3.857

3.  Heneicosapentaenoate (21:5n-3): its incorporation into lipids and its effects on arachidonic acid and eicosanoid synthesis.

Authors:  L N Larsen; K Høvik; J Bremer; K H Holm; F Myhren; B Børretzen
Journal:  Lipids       Date:  1997-07       Impact factor: 1.880

4.  Expression of peroxisome proliferator-activated receptors in zebrafish (Danio rerio) depending on gender and developmental stage.

Authors:  Arantza Ibabe; Eider Bilbao; Miren P Cajaraville
Journal:  Histochem Cell Biol       Date:  2004-12-23       Impact factor: 4.304

5.  Isolation of peroxisomal enoyl-CoA hydratase in rainbow trout and immunochemical identification with the bifunctional enzyme.

Authors:  L A Baldwin; E J Calabrese; P T Kostecki; J H Yang
Journal:  Fish Physiol Biochem       Date:  1990-07       Impact factor: 2.794

6.  Angiotensin-(1-7) prevents diabetes-induced attenuation in PPAR-gamma and catalase activities.

Authors:  Gursev S Dhaunsi; Mariam H M Yousif; Saghir Akhtar; Mark C Chappell; Debra I Diz; Ibrahim F Benter
Journal:  Eur J Pharmacol       Date:  2010-05-04       Impact factor: 4.432

7.  Short- and long-term responses and recovery of mussels Mytilus edulis exposed to heavy fuel oil no. 6 and styrene.

Authors:  Pamela Ruiz; Maren Ortiz-Zarragoitia; Amaia Orbea; Sjur Vingen; Anne Hjelle; Thierry Baussant; Miren P Cajaraville
Journal:  Ecotoxicology       Date:  2014-03-28       Impact factor: 2.823

8.  Rats selectively bred for low aerobic capacity have reduced hepatic mitochondrial oxidative capacity and susceptibility to hepatic steatosis and injury.

Authors:  John P Thyfault; R Scott Rector; Grace M Uptergrove; Sarah J Borengasser; E Matthew Morris; Yongzhong Wei; Matt J Laye; Charles F Burant; Nathan R Qi; Suzanne E Ridenhour; Lauren G Koch; Steve L Britton; Jamal A Ibdah
Journal:  J Physiol       Date:  2009-02-23       Impact factor: 5.182

9.  Acute, but not chronic, leptin treatment induces acyl-CoA oxidase in C2C12 myotubes.

Authors:  Roberta Ceci; Stefania Sabatini; Guglielmo Duranti; Isabella Savini; Luciana Avigliano; Antonello Rossi
Journal:  Eur J Nutr       Date:  2007-06-14       Impact factor: 5.614

10.  Sesamin increases alpha-linolenic acid conversion to docosahexaenoic acid in atlantic salmon (Salmo salar L.) hepatocytes: role of altered gene expression.

Authors:  Sofia Trattner; B Ruyter; T K Østbye; T Gjøen; V Zlabek; A Kamal-Eldin; J Pickova
Journal:  Lipids       Date:  2008-09-11       Impact factor: 1.880
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