Literature DB >> 39946

Enzyme-linked immunosorbent assay for detection of Streptococcus pneumoniae antigen.

S A Harding, W M Scheld, M D McGowan, M A Sande.   

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Streptococcus pneumoniae polysaccharide antigen in cerebrospinal fluid and serum. Sensitivity and specificity were determined for purified antigen preparations. Specificity was also evaluated in the rabbit meningitis model, and the sensitivity was compared to counterimmunoelectrophoresis, using the infected rabbits' cerebrospinal fluid and serum. The ELISA was a specific technique for detecting S. pneumoniae antigen. ELISA was 25 times more sensitive than counterimmunoelectrophoresis for purified antigen and resulted in an increased positivity of the cerebrospinal fluid and serum from infected rabbits. ELISA should prove very useful in the diagnosis of pneumococcal infections.

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Year:  1979        PMID： 39946      PMCID： PMC273165          DOI： 10.1128/jcm.10.3.339-342.1979

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

1.  Detection of bacterial antigen in pleural fluid by counterimmunoelectrophoresis.

Authors:  R M Lampe; T Chottipitayasunondh; P Sunakorn
Journal:  J Pediatr       Date:  1976-04       Impact factor: 4.406

2.  Detection of viral proteins in mouse mammary tumors by immunoperoxidase staining of paraffin sections.

Authors:  I Keydar; R Mesa-Tejada; M Ramanarayanan; T Ohno; C Fenoglio; R Hu; S Spiegelman
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205

3.  Diagnosis of pneumococcal pneumonia by antigen detection in sputum.

Authors:  J Miller; M A Sande; J M Gwaltney; J O Hendley
Journal:  J Clin Microbiol       Date:  1978-05       Impact factor: 5.948

4.  Detection of pneumococcal polysaccharide in the sputum of patients with pneumococcal pneumonia by counterimmunoelectrophoresis.

Authors:  C A Perlino; J A Shulman
Journal:  J Lab Clin Med       Date:  1976-03

5.  Counterimmunoelectrophoresis for rapid diagnosis of meningitis due to Diplococcus pneumoniae.

Authors:  B Fossieck; R Craig; P Y Paterson
Journal:  J Infect Dis       Date:  1973-01       Impact factor: 5.226

6.  Omni-serum. A diagnostic Pneumococcus serum, reacting with the 82 known types of Pneumococcus.

Authors:  E Lund; P Rasmussen
Journal:  Acta Pathol Microbiol Scand       Date:  1966

7.  Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.

Authors:  E Engvall; P Perlmann
Journal:  J Immunol       Date:  1972-07       Impact factor: 5.422

8.  Cefamandole and ampicillin therapy in experimental Haemophilus influenzae meningitis.

Authors:  L J Strausbaugh; C D Mandaleris; M A Sande
Journal:  J Infect Dis       Date:  1977-02       Impact factor: 5.226

9.  Pneumococcal antigen in lobar pneumonia.

Authors:  P Tugwell; B M Greenwood
Journal:  J Clin Pathol       Date:  1975-02       Impact factor: 3.411

10.  Effect of probenecid on cerebrospinal fluid concentrations of penicillin and cephalosporin derivatives.

Authors:  R G Dacey; M A Sande
Journal:  Antimicrob Agents Chemother       Date:  1974-10       Impact factor: 5.191
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  10 in total

1.  A reassessment of sputum specimens for laboratory diagnosis of pneumococcal pneumonia.

Authors:  A Balows
Journal:  Eur J Clin Microbiol       Date:  1986-06       Impact factor: 3.267

2.  Indirect sandwich enzyme-linked immunosorbent assay for rapid detection of Streptococcus pneumoniae type 3 antigen.

Authors:  D L Drow; D D Manning
Journal:  J Clin Microbiol       Date:  1980-06       Impact factor: 5.948

3.  Rapid diagnosis of bacterial meningitis by an enzyme immunoassay of cerebrospinal fluid.

Authors:  M A Salih; H S Ahmed; Y Hofvander; D Danielsson; P Olcén
Journal:  Epidemiol Infect       Date:  1989-10       Impact factor: 2.451

4.  Detection of Neisseria meningitidis group A, Haemophilus influenzae type b, and Streptococcus pneumoniae antigens in cerebrospinal fluid specimens by antigen capture enzyme-linked immunosorbent assays.

Authors:  J E Sippel; C M Prato; N I Girgis; E A Edwards
Journal:  J Clin Microbiol       Date:  1984-08       Impact factor: 5.948

5.  Rapid diagnosis of severe Haemophilus influenzae serotype b infections by monoclonal antibody enzyme immunoassay for outer membrane proteins.

Authors:  A Belmaaza; J Hamel; S Mousseau; S Montplaisir; B R Brodeur
Journal:  J Clin Microbiol       Date:  1986-09       Impact factor: 5.948

6.  Routine use of counterimmunoelectrophoresis for the detection of pneumococcal antigen in sputum.

Authors:  C H Ericsson; H O Hallander; A Rosen; A M Sjögren; I Sjögren
Journal:  Med Microbiol Immunol       Date:  1986       Impact factor: 3.402

7.  Comparison of enzyme-linked immunosorbent assay with coagglutination and latex agglutination for rapid diagnosis of pneumococcal pneumonia by detecting antigen in sputa.

Authors:  H Holmberg; A Krook
Journal:  Eur J Clin Microbiol       Date:  1986-06       Impact factor: 3.267

8.  Comparison of immunological methods for diagnosis of pneumococcal pneumonia in biological fluids.

Authors:  S Lenthe-Eboa; G Brighouse; R Auckenthaler; D Lew; A Zwahlen; P H Lambert; F A Waldvogel
Journal:  Eur J Clin Microbiol       Date:  1987-02       Impact factor: 3.267

9.  Identification of Streptococcus pneumoniae by the Phadebact coagglutination test.

Authors:  N M Burdash; M E West
Journal:  J Clin Microbiol       Date:  1982-03       Impact factor: 5.948

10.  Principles of nucleic acid hybridization and comparison with monoclonal antibody technology for the diagnosis of infectious diseases.

Authors:  S C Edberg
Journal:  Yale J Biol Med       Date:  1985 Sep-Oct
  10 in total

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