Literature DB >> 3993931

A single affinity column step method for the purification of ricin toxin from castor beans (Ricinus communis).

B M Simmons, J H Russell.   

Abstract

A rapid method for purifying ricin toxin from castor beans is presented which uses a single affinity column step to obtain pure toxin from a crude extract of castor beans. A galactosyl-Sepharose affinity matrix was used to bind ricin toxin and its associated agglutinin, which both bind specifically to galactose, from a crude extract. The selective elution of ricin toxin and agglutinin was then achieved by eluting the affinity column with a galactose gradient, which sequentially elutes the two proteins due to a difference in binding avidity to the matrix.

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Year:  1985        PMID： 3993931     DOI： 10.1016/0003-2697(85)90417-8

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  8 in total

1.  High-Definition Mapping of Four Spatially Distinct Neutralizing Epitope Clusters on RiVax, a Candidate Ricin Toxin Subunit Vaccine.

Authors:  Ronald T Toth; Siva Krishna Angalakurthi; Greta Van Slyke; David J Vance; John M Hickey; Sangeeta B Joshi; C Russell Middaugh; David B Volkin; David D Weis; Nicholas J Mantis
Journal:  Clin Vaccine Immunol       Date:  2017-12-05

2.  Comparative glycoproteomics of stem cells identifies new players in ricin toxicity.

Authors:  Johannes Stadlmann; Jasmin Taubenschmid; Daniel Wenzel; Anna Gattinger; Gerhard Dürnberger; Frederico Dusberger; Ulrich Elling; Lukas Mach; Karl Mechtler; Josef M Penninger
Journal:  Nature       Date:  2017-09-20       Impact factor: 49.962

3.  A lyophilized formulation of RiVax, a recombinant ricin subunit vaccine, retains immunogenicity.

Authors:  Joan E Smallshaw; Ellen S Vitetta
Journal:  Vaccine       Date:  2010-01-12       Impact factor: 3.641

4.  A vital sugar code for ricin toxicity.

Authors:  Jasmin Taubenschmid; Johannes Stadlmann; Markus Jost; Tove Irene Klokk; Cory D Rillahan; Andreas Leibbrandt; Karl Mechtler; James C Paulson; Julian Jude; Johannes Zuber; Kirsten Sandvig; Ulrich Elling; Thorsten Marquardt; Christian Thiel; Christian Koerner; Josef M Penninger
Journal:  Cell Res       Date:  2017-09-19       Impact factor: 25.617

5.  Comparison of microbial and transient expression (tobacco plants and plant-cell packs) for the production and purification of the anticancer mistletoe lectin viscumin.

Authors:  Benjamin B Gengenbach; Linda L Keil; Patrick Opdensteinen; Catherine R Müschen; Georg Melmer; Hans Lentzen; Jens Bührmann; Johannes F Buyel
Journal:  Biotechnol Bioeng       Date:  2019-06-29       Impact factor: 4.530

6.  Mucoricin is a ricin-like toxin that is critical for the pathogenesis of mucormycosis.

Authors:  Sameh S M Soliman; Clara Baldin; Yiyou Gu; Shakti Singh; Teclegiorgis Gebremariam; Marc Swidergall; Abdullah Alqarihi; Eman G Youssef; Sondus Alkhazraji; Antonis Pikoulas; Christina Perske; Vivek Venkataramani; Abigail Rich; Vincent M Bruno; Julie Dunning Hotopp; Nicolas J Mantis; John E Edwards; Scott G Filler; Georgios Chamilos; Ellen S Vitetta; Ashraf S Ibrahim
Journal:  Nat Microbiol       Date:  2021-01-18       Impact factor: 17.745

7.  Characterization of the binding properties and retrograde axonal transport of a monoclonal antibody directed against the rat nerve growth factor receptor.

Authors:  M Taniuchi; E M Johnson
Journal:  J Cell Biol       Date:  1985-09       Impact factor: 10.539

8.  Selective destruction of nerve growth factor receptor-bearing cells in vitro using a hybrid toxin composed of ricin A chain and a monoclonal antibody against the nerve growth factor receptor.

Authors:  P S DiStefano; J B Schweitzer; M Taniuchi; E M Johnson
Journal:  J Cell Biol       Date:  1985-09       Impact factor: 10.539
  8 in total

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